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PMID: 8764085 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Analysis of conserved domains of UL41 of herpes simplex virus type 1 in virion host shutoff and pathogenesis.

Journal of virology ·Vol. 70 ·No. 8 ·1996-08-00 ·Pages 5665-7

Strelow LI, Leib DA

Abstract

The herpes simplex virus type 1 virion host shutoff protein has four domains whose sequences are conserved only among neurotropic herpesviruses. Mutant viruses with 29- and 31-amino-acid deletions in domains III and IV but outside of the domain required for interaction with VP16 were generated. The mutants failed to induce cellular RNA degradation and showed impaired virulence in mice. Domains III and IV are therefore required for both shutoff and virulence.

MeSH Terms
Animals Chlorocebus aethiops Herpes Simplex/virology Herpes Simplex Virus Protein Vmw65/metabolism Mice Mutation RNA, Viral/genetics,metabolism Simplexvirus/genetics,metabolism,pathogenicity Vero Cells Viral Proteins/genetics,metabolism Virulence
Chemicals
Herpes Simplex Virus Protein Vmw65 RNA, Viral Ul41 protein, Human herpesvirus 1 Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Strelow L I
Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Leib D A
References (24)
24 references, click to expand
  1. Degradation of cellular mRNA during infection by herpes simplex virus.
    Proc Natl Acad Sci U S A. 1977 Jun;74(6):2370-4 PMID: 196289
  2. The virion host shutoff protein of herpes simplex virus type 1: messenger ribonucleolytic activity in vitro.
    J Virol. 1996 Apr;70(4):2411-9 PMID: 8642669
  3. Suppression of the synthesis of cellular macromolecules by herpes simplex virus.
    J Gen Virol. 1978 Oct;41(1):37-51 PMID: 212520
  4. Anatomy of herpes simplex virus DNA. XI. Apparent clustering of functions effecting rapid inhibition of host DNA and protein synthesis.
    J Virol. 1979 Feb;29(2):825-7 PMID: 219262
  5. Early and delayed shut-off of host protein synthesis in cells infected with herpes simplex virus.
    J Gen Virol. 1982 Jul;61 (Pt l):121-5 PMID: 6288847
  6. Herpes simplex virus mutants defective in the virion-associated shutoff of host polypeptide synthesis and exhibiting abnormal synthesis of alpha (immediate early) viral polypeptides.
    J Virol. 1983 May;46(2):498-512 PMID: 6302315
  7. Early virion-associated suppression of cellular protein synthesis by herpes simplex virus is accompanied by inactivation of mRNA.
    J Gen Virol. 1984 Jul;65 ( Pt 7):1225-8 PMID: 6086815
  8. Herpes simplex virus-infected cells contain a function(s) that destabilizes both host and viral mRNAs.
    Proc Natl Acad Sci U S A. 1987 Apr;84(7):1926-30 PMID: 3031658
  9. Effects of herpes simplex virus on mRNA stability.
    J Virol. 1987 Jul;61(7):2198-207 PMID: 3035220
  10. Herpes simplex virus virion host shutoff function.
    J Virol. 1988 Mar;62(3):912-21 PMID: 2828686
  11. The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1.
    J Gen Virol. 1988 Jul;69 ( Pt 7):1531-74 PMID: 2839594
  12. A herpes simplex virus type 1 mutant containing a nontransinducing Vmw65 protein establishes latent infection in vivo in the absence of viral replication and reactivates efficiently from explanted trigeminal ganglia.
    J Virol. 1990 Apr;64(4):1630-8 PMID: 2157048
  13. Differential regulation of endogenous and transduced beta-globin genes during infection of erythroid cells with a herpes simplex virus type 1 recombinant.
    J Virol. 1990 Aug;64(8):3882-94 PMID: 1695257
  14. Quantitative polymerase chain reaction analysis of herpes simplex virus DNA in ganglia of mice infected with replication-incompetent mutants.
    J Virol. 1990 Sep;64(9):4288-95 PMID: 2166818
  15. Inactivation of the shutoff gene (UL41) of herpes simplex virus types 1 and 2.
    J Gen Virol. 1990 Dec;71 ( Pt 12):2961-7 PMID: 2177088
  16. Identification and characterization of the virion-induced host shutoff product of herpes simplex virus gene UL41.
    J Gen Virol. 1992 Feb;73 ( Pt 2):467-70 PMID: 1311370
  17. The pseudorabies virus host-shutoff homolog gene: nucleotide sequence and comparison with alphaherpesvirus protein counterparts.
    Virology. 1993 Apr;193(2):1028-32 PMID: 8384744
  18. Isolation of a herpes simplex virus type 1 mutant with a deletion in the virion host shutoff gene and identification of multiple forms of the vhs (UL41) polypeptide.
    J Virol. 1993 Dec;67(12):7149-60 PMID: 8230437
  19. Herpes simplex virus VP16 forms a complex with the virion host shutoff protein vhs.
    J Virol. 1994 Apr;68(4):2339-46 PMID: 8139019
  20. Mutational analysis of the herpes simplex virus virion host shutoff protein: evidence that vhs functions in the absence of other viral proteins.
    J Virol. 1995 Aug;69(8):4863-71 PMID: 7609054
  21. The virion host shutoff protein of herpes simplex virus inhibits reporter gene expression in the absence of other viral gene products.
    Virology. 1995 Aug 20;211(2):491-506 PMID: 7645253
  22. Role of the virion host shutoff (vhs) of herpes simplex virus type 1 in latency and pathogenesis.
    J Virol. 1995 Nov;69(11):6779-86 PMID: 7474089
  23. Identification and characterization of a small modular domain in the herpes simplex virus host shutoff protein sufficient for interaction with VP16.
    J Virol. 1996 Apr;70(4):2124-31 PMID: 8642633
  24. Alterations in the protein synthetic apparatus of Friend erythroleukemia cells infected with vesicular stomatitis virus or herpes simplex virus.
    J Virol. 1978 Jan;25(1):422-6 PMID: 202753
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.8.5665-5667.1996
Published
1996-08-00
Pages
5665-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC190531
Subset
IM
Grants
NEI NIH HHS · R01 EY010707 · United States
NEI NIH HHS · P30-EY02687 · United States
NEI NIH HHS · R01 EY10707 · United States
External Links
PubMed source DOI: 10.1128/JVI.70.8.5665-5667.1996 PMC Full Text View journal details
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