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PMID: 8755898 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Spontaneous and induced mutations in a single open reading frame alter both virulence and avirulence in Xanthomonas campestris pv. vesicatoria avrBs2.

Journal of bacteriology ·Vol. 178 ·No. 15 ·1996-08-00 ·Pages 4661-9

Swords KM, Dahlbeck D, Kearney B, Roy M, Staskawicz BJ

Abstract

Molecular characterization of the avrBs2 locus from Xanthomonas campestris pv. vesicatoria has revealed that expression of this gene triggers disease resistance in Bs2 pepper (Capsicum annuum) plants and contributes to virulence of the pathogen. Deletion analysis and site-directed mutagenesis established the avrBs2 gene as a 2,190-bp open reading frame encoding a putative 80.1-kDa protein. Two classes of Xanthomonas pathogens evading Bs2 host resistance and displaying reduced fitness were found to be specifically mutated in avrBs2. Members of one class contained a 5-bp insertion, while the second class was distinguished by a divergent 3' region of avrBs2; both mutant classes were complemented in trans by a plasmid-borne copy of avrBs2. A divergent avrBs2 homolog was cloned from the Brassica pathogen X. campestris pv. campestris. The predicted AvrBs2 proteins from the two Xanthomonas pathovars were strongly conserved and had predicted sequence similarity with both Agrobacterium tumefaciens agrocinopine synthase and Escherichia coli UgpQ, two enzymes involved in the synthesis or hydrolysis of phosphodiester linkages. On the basis of homology with agrocinopine synthase and UgpQ and the dual phenotype of avirulence and virulence, several models for the function of AvrBs2 are proposed.

MeSH Terms
Alleles Amino Acid Sequence Base Sequence Capsicum/microbiology DNA Primers/genetics DNA, Bacterial/genetics Genes, Bacterial Genetic Complementation Test Molecular Sequence Data Mutagenesis, Site-Directed Mutation Open Reading Frames Phenotype Plants, Medicinal Sequence Deletion Sequence Homology, Amino Acid Virulence/genetics Xanthomonas campestris/genetics,pathogenicity
Chemicals
DNA Primers DNA, Bacterial
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Swords K M
Department of Plant Biology, University of California, Berkeley 94720, USA.
Dahlbeck D
Kearney B
Roy M
Staskawicz B J
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.15.4661-4669.1996
Published
1996-08-00
Pages
4661-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC178237
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.178.15.4661-4669.1996 PMC Full Text View journal details
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