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PMID: 8742709 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Ovule development: identification of stage-specific and tissue-specific cDNAs.

The Plant cell ·Vol. 8 ·No. 2 ·1996-02-00 ·Pages 213-39

Nadeau JA, Zhang XS, Li J, O'Neill SD

Abstract

A differential screening approach was used to identify seven ovule-specific cDNAs representing genes that are expressed in a stage-specific manner during ovule development. The Phalaenopsis orchid takes 80 days to complete the sequence of ovule developmental events, making it a good system to isolate stage-specific ovule genes. We constructed cDNA libraries from orchid ovule tissue during archesporial cell differentiation, megasporocyte formation, and the transition to meiosis, as well as during the final mitotic divisions of female gametophyte development. RNA gel blot hybridization analysis revealed that four clones were stage specific and expressed solely in ovule tissue, whereas one clone was specific to pollen tubes. Two other clones were not ovule specific. Sequence analysis and in situ hybridization revealed the identities and domain of expression of several of the cDNAs. O39 encodes a putative homeobox transcription factor that is expressed early in the differentiation of the ovule primordium; O40 encodes a cytochrome P450 monooxygenase (CYP78A2) that is pollen tube specific. O108 encodes a protein of unknown function that is expressed exclusively in the outer layer of the outer integument and in the female gametophyte of mature ovules. O126 encodes a glycine-rich protein that is expressed in mature ovules, and O141 encodes a cysteine proteinase that is expressed in the outer integument of ovules during seed formation. Sequences homologous to these ovule clones can now be isolated from other organisms, and this should facilitate their functional characterization.

MeSH Terms
Amino Acid Sequence Base Sequence Consensus Sequence Cysteine Endopeptidases/biosynthesis,genetics Cytochrome P-450 Enzyme System/biosynthesis,chemistry,genetics DNA, Complementary/analysis Gene Expression Genes, Homeobox Genes, Plant In Situ Hybridization Molecular Sequence Data Organ Specificity Phylogeny Plant Physiological Phenomena Plant Proteins/biosynthesis,genetics Plants/genetics Seeds/physiology Sequence Homology, Amino Acid Transcription Factors/biosynthesis,genetics Transcription, Genetic
Chemicals
DNA, Complementary Plant Proteins Transcription Factors Cytochrome P-450 Enzyme System Cysteine Endopeptidases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nadeau J A
Division of Biological Sciences, University of California at Davis 95616, USA.
Zhang X S
Li J
O'Neill S D
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.8.2.213
Published
1996-02-00
Pages
213-39
Language
English
Region
England
NLM ID
9208688
PMCID
PMC161093
Subset
IM
Databases
GENBANK
U34743, U34744, U34745, U34746, U34747
External Links
PubMed source DOI: 10.1105/tpc.8.2.213 PMC Full Text View journal details
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