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PMID: 8049361 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Isolation of a cytochrome P450 homologue preferentially expressed in developing inflorescences of Zea mays.

Plant molecular biology ·Vol. 25 ·No. 3 ·1994-06-00 ·Pages 343-53

Larkin JC

Abstract

Four cDNA clones exhibiting preferential hybridization to transcripts present in developing maize tassels were isolated by differential screening. One of these cDNA clones hybridizes to transcripts detectable only in the shoot apex. The abundance of this transcript is significantly higher in developing inflorescence apices than in vegetative apices. DNA sequence analysis of a 2107 nucleotide cDNA clone corresponding to this transcript revealed that the transcript encodes a polypeptide of 547 amino acids, with a molecular mass of 58.4 kDa. This polypeptide shares significant sequence similarity with members of the cytochrome P450 monooxygenase gene superfamily, including the conserved C-terminal domains typical of the cytochrome P450 monooxygenases.

Related Genes
CYP-78 ZMAY
MeSH Terms
Amino Acid Sequence Cytochrome P-450 Enzyme System/genetics DNA, Complementary/genetics Gene Library Genes, Plant/genetics Molecular Sequence Data Plant Proteins Reproduction Sequence Analysis, DNA Sequence Homology, Amino Acid Tissue Distribution Zea mays/enzymology,genetics,growth & development
Chemicals
DNA, Complementary Plant Proteins Cytochrome P-450 Enzyme System CYP78A1 protein, Zea mays
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Larkin J C
Department of Biochemistry, University of Minnesota, St. Paul 55108.
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36 references, click to expand
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00043864
Published
1994-06-00
Pages
343-53
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
L23209
External Links
PubMed source DOI: 10.1007/BF00043864 View journal details
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