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PMID: 874085 Published · ppublish English Journal Article

Prostaglandin biosynthesis by rabbit renomedullary interstitial cells in tissue culture. Stimulation by angiotensin II, bradykinin, and arginine vasopressin.

The Journal of clinical investigation ·Vol. 60 ·No. 1 ·1977-07-00 ·Pages 215-23

Zusman RM, Keiser HR

Abstract

Rabbit renomedullary interstitial cells were isolated and grown in tissue culture. These cells synthesize 0.8 ng of prostaglandin E2 (PGE2) per microgram cellular protein per hour in monolayer tissue culture; prostaglandins A2 and F2alpha (PGA2 and PGF2alpha) biosynthesis was 10 and 5% of PGE2 biosynthesis, respectively. Arachidonic acid markedly stimulated the production of PGE2 and PGF2alpha, with conversion rates of 0.24 and 0.02%/h, respectively. Angiotensin II, hyperosmolality, bradykinin, and arginine vasopressin each stimulated PGE2 biosynthesis; maximum stimulation was 20, 3.7, 3.6, and 3.2 times basal production, respectively. PGE2 biosynthesis by the renomedullary interstitial cells was inhibited by isoproterenol, potassium, nonsteroidal anti-inflammatory agents (indomethacin, naproxen, ibuprofen, suprofen, meclofenamate, and acetylsalicylic acid), mepacrine (a phospholipase inhibitor), hydrocortisone, and cortisone. The rabbit renomedullary interstitial cell in tissue culture is a model system for the study of hormonal regulation of PGE2 biosynthesis.

MeSH Terms
Angiotensin II/pharmacology Animals Arachidonic Acids/pharmacology Arginine Vasopressin/pharmacology Bradykinin/pharmacology Cell Division Cells, Cultured Kidney/metabolism Kidney Medulla/cytology,drug effects,metabolism Osmolar Concentration Potassium/pharmacology Prostaglandins/biosynthesis Prostaglandins A/biosynthesis Prostaglandins E/biosynthesis Prostaglandins F/biosynthesis Rabbits Sodium/pharmacology Structure-Activity Relationship Vasopressins/analogs & derivatives
Chemicals
Arachidonic Acids Prostaglandins Prostaglandins A Prostaglandins E Prostaglandins F Vasopressins Angiotensin II Arginine Vasopressin Sodium Potassium Bradykinin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zusman R M
Keiser H R
References (46)
46 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI108758
Published
1977-07-00
Pages
215-23
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC372359
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI108758 PMC Full Text View journal details
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