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PMID: 8730097 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

SDC25, a dispensable Ras guanine nucleotide exchange factor of Saccharomyces cerevisiae differs from CDC25 by its regulation.

Molecular biology of the cell ·Vol. 7 ·No. 4 ·1996-04-00 ·Pages 529-39

Boy-Marcotte E, Ikonomi P, Jacquet M

Abstract

The SDC25 gene of Saccharomyces cerevisiae is homologous to CDC25. Its 3' domain encodes a guanine nucleotide exchange factor (GEF) for Ras. Nevertheless, the GEF encoded by CDC24 is determinant for the Ras/cAMP pathway activation in growth. We demonstrate that the SDC25 gene product is a functional GEF for Ras: the complete SDC25 gene functionally replaces CDC25 when overexpressed or when transcribed under CDC25 transcriptional control at the CDC25 locus. Chimeric proteins between Sdc25p and Cdc25p are also functional GEFs for Ras. We also show that the two genes are differentially regulated: SDC25 is not transcribed at a detectable level in growth conditions when glucose is the carbon source. It is transcribed at the end of growth when nutrients are depleted and in cells grown on nonfermentable carbon sources. In contrast, CDC25 accumulation is slightly reduced when glucose is replaced by a nonfermentable carbon source.

MeSH Terms
Base Sequence Blotting, Northern Cell Cycle Proteins/genetics,physiology Fungal Proteins/genetics,physiology GTP-Binding Proteins/genetics,physiology Gene Expression Regulation, Fungal Molecular Sequence Data Open Reading Frames/genetics Phenotype Saccharomyces cerevisiae/genetics Transcription Factors/genetics,physiology Transcription, Genetic rap GTP-Binding Proteins ras-GRF1
Chemicals
Cell Cycle Proteins Fungal Proteins Transcription Factors ras-GRF1 GTP-Binding Proteins rap GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Boy-Marcotte E
Institut de Génétique et Microbiologie, URA Centre National de la Recherche Scientifique D1354, Université Paris XI, Orsay, France.
Ikonomi P
Jacquet M
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.7.4.529
Published
1996-04-00
Pages
529-39
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC275907
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.7.4.529 PMC Full Text View journal details
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