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PMID: 8727873 Published · ppublish English Journal Article

Evaluation of tuberculosis transmission in a community by 1 year of systematic typing of Mycobacterium tuberculosis clinical isolates.

Journal of clinical microbiology ·Vol. 34 ·No. 5 ·1996-05-00 ·Pages 1043-9

Torrea G, Offredo C, Simonet M, Gicquel B, Berche P, Pierre-Audigier C

Abstract

Interhuman transmission of Mycobacterium tuberculosis was investigated by using molecular typing, including restriction fragment length polymorphism with probes IS6110, DR (direct repeat) and PGRS (polymorphic GC-rich sequence) and a PCR method using the inverted repeat sequences of IS6110 as primers. From 105 patients hospitalized for tuberculosis during a 1-year survey in three hospitals in Paris, France, 111 isolates were collected and analyzed. Eighty-eight patients were infected with genetically different isolates, demonstrating the clonal heterogeneity of M. tuberculosis in these patients originating from various geographical areas. Fourteen patients were infected by strains clustered with identical fingerprints. An epidemiological relatedness was demonstrated for isolates from only seven of these patients. Thus, the typing of isolates from all tuberculous patients in hospitals during 1 year allows the detection of transmission in the general community. This would improve the case findings, thereby further improving the detection of outbreaks.

MeSH Terms
Adult Bacterial Typing Techniques Base Sequence DNA Primers/genetics DNA Transposable Elements DNA, Bacterial/genetics Disease Outbreaks Drug Resistance, Microbial Female Humans Male Middle Aged Molecular Epidemiology Molecular Sequence Data Mycobacterium tuberculosis/classification,genetics,isolation & purification Paris/epidemiology Polymerase Chain Reaction Polymorphism, Genetic Polymorphism, Restriction Fragment Length Repetitive Sequences, Nucleic Acid Tuberculosis/epidemiology,microbiology,transmission
Chemicals
DNA Primers DNA Transposable Elements DNA, Bacterial
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Torrea G
Unité de Génétique Mycobactérienne, Institut Pasteur, Paris, France.
Offredo C
Simonet M
Gicquel B
Berche P
Pierre-Audigier C
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.34.5.1043-1049.1996
Published
1996-05-00
Pages
1043-9
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC228952
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.34.5.1043-1049.1996 PMC Full Text View journal details
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