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PMID: 7710330 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of the highly abundant polymorphic GC-rich-repetitive sequence (PGRS) present in Mycobacterium tuberculosis.

Archives of microbiology ·Vol. 163 ·No. 2 ·1995-02-00 ·Pages 87-95

Poulet S, Cole ST

Abstract

The polymorphic GC-rich repetitive sequence (PGRS) found on the chromosome of Mycobacterium tuberculosis was characterized by means of mapping, cloning and sequencing. PGRS was present in at least 26 loci and consisted of many tandem repeats of the consensus sequence CGGCGGCAA. As the core of the consensus motif was the triplet CGC, or CRR (where R is a purine), it seems likely that PGRS arose by means of triplet expansion, accounting for its polymorphism. Several copies of PGRS were linked to a conserved open reading frame. PGRS was used as the target sequence for the polymerase chain reaction in an attempt to develop a new typing technique.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Mapping Chromosomes, Bacterial Cloning, Molecular Consensus Sequence/genetics DNA, Bacterial/analysis,genetics Molecular Sequence Data Mycobacterium/genetics Mycobacterium tuberculosis/genetics Open Reading Frames/genetics Polymerase Chain Reaction/methods Polymorphism, Restriction Fragment Length Repetitive Sequences, Nucleic Acid/genetics Sequence Alignment Sequence Analysis, DNA
Chemicals
DNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Poulet S
Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, Paris, France.
Cole S T
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Article Info
Journal
Archives of microbiology
Abbr.
Arch Microbiol
ISSN
0302-8933
DOI
10.1007/BF00381781
Published
1995-02-00
Pages
87-95
Language
English
Region
Germany
NLM ID
0410427
Subset
IM
Databases
GENBANK
S76843
External Links
PubMed source DOI: 10.1007/BF00381781 View journal details
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