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PMID: 8722782 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Effects of single P-element insertions on olfactory behavior in Drosophila melanogaster.

Genetics ·Vol. 143 ·No. 1 ·1996-05-00 ·Pages 293-301

Anholt RR, Lyman RF, Mackay TF

Abstract

Single P-element (P[lArB]) insertional mutagenesis of an isogenic strain was used to identify autosomal loci affecting odor-guided behavior of Drosophila melanogaster. The avoidance response to benzaldehyde of 379 homozygous P[lArB] element-containing insert lines was evaluated quantitatively. Fourteen smell impaired (smi) lines were identified in which P[lArB] element insertion caused different degrees of hyposmia in one or both sexes. The smi loci map to different cytological locations and probably are novel olfactory genes. Enhancer trap analysis of the smi lines indicates that expression of at least 10 smi genes is controlled by olfactory tissue-specific promoter/enhancer elements.

MeSH Terms
Animals Avoidance Learning Behavior, Animal Benzaldehydes Chromosome Mapping DNA Transposable Elements Drosophila melanogaster/genetics,physiology In Situ Hybridization Larva Mutagenesis, Insertional Odorants Salivary Glands Smell
Chemicals
Benzaldehydes DNA Transposable Elements benzaldehyde
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Anholt R R
Department of Zoology, North Carolina State University, Raleigh 27695, USA.
Lyman R F
Mackay T F
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/143.1.293
Published
1996-05-00
Pages
293-301
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1207262
Subset
IM
Grants
NIDCD NIH HHS · DC-02485 · United States
NIGMS NIH HHS · GM-45146 · United States
NIGMS NIH HHS · GM-45344 · United States
External Links
PubMed source DOI: 10.1093/genetics/143.1.293 PMC Full Text View journal details
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