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PMID: 87190 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Detection, purification and characterization of a human cancer-associated galactosyltransferase acceptor.

The Biochemical journal ·Vol. 178 ·No. 2 ·1979-02-15 ·Pages 279-87

Podolsky DK, Weiser MM

Abstract

A low-molecular-weight acceptor of galactosyltransferase activity was detected in sera and effusions of patients with extensive maligant disease. This substance was purified to homogeneity from both human serum and effusion by using sequential charcoal/Celite and DEAE-cellulose column chromatography. The purified acceptor was shown to act as substrate for both purified normal and cancer-associated human galactosyltransferase (EC 2.4.1.22) isoenzymes, but had a higher affinity for the cancer-associated isoenzyme (Km = 20 microM) than for the normal isoenzyme (Km = 500 microM). The substrate was found to be a glycopeptide with mol.wt. approx. 3600 determined by polyacrylamide-gel chromatography. Carbohyydate analysis demonstrated only the presence of glucosamine and mannose. Amino acid analysis revealed that the peptide moiety consisted of eight different amino acids, including two residues of asparagine and one residue of serine, but no threonine. These structural data suggest that the acceptor is a fraction of an asparagine-glucosamine type of glycoprotein.

MeSH Terms
Amino Acids/analysis Carbohydrates/analysis Galactosyltransferases/metabolism Glycopeptides/isolation & purification,pharmacology Humans Isoenzymes/metabolism Kinetics Neoplasms/analysis,enzymology Uridine Diphosphate Galactose alpha-Fetoproteins
Chemicals
Amino Acids Carbohydrates Glycopeptides Isoenzymes alpha-Fetoproteins Uridine Diphosphate Galactose Galactosyltransferases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Podolsky D K
Weiser M M
References (44)
44 references, click to expand
  1. Immunosuppressive and immunostimulatory factors produced by malignant cells in vitro.
    Int J Cancer. 1974 Apr 15;13(4):530-42 PMID: 4601698
  2. Cell-surface glycoproteins of two sublines of the TA3 tumor.
    J Natl Cancer Inst. 1973 Aug;51(2):585-91 PMID: 4797260
  3. Glycosyltransferases in human blood. II. Study of serum galactosyltransferase and N-acetylgalactosaminyltransferase in patients with liver diseases.
    J Clin Invest. 1972 Aug;51(8):2033-9 PMID: 5054462
  4. The synthesis of complex carbohydrates by multiglycosyltransferase systems and their potential function in intercellular adhesion.
    Chem Phys Lipids. 1970 Oct;5(1):270-97 PMID: 5476326
  5. Gas-liquid chromatography of amino acids in biological substances.
    J Chromatogr. 1970 Dec;53(2):171-94 PMID: 5498839
  6. The effects on cell multiplication in vitro of a dialysable polypeptide derived from tumor fluids.
    Eur J Cancer. 1968 Jul;4(3):271-7 PMID: 5760802
  7. On the structure and biological effects of a newly-discovered cytotoxic polypeptide in tumor fluid.
    Eur J Cancer. 1965 Nov;1(3):199-202 PMID: 5874714
  8. A factor toxic to chick embryos in blood of tumor bearing animals.
    Eur J Cancer. 1966 Aug;2(3):205-9 PMID: 5974756
  9. Glycoprortein biosynthesis in small intestinal mucosa. I. A study of glycosyltransferases in microsomal subfractions.
    J Biol Chem. 1971 Sep 10;246(17):5466-76 PMID: 10970180
  10. The thiobarbituric acid assay of sialic acids.
    J Biol Chem. 1959 Aug;234(8):1971-5 PMID: 13672998
  11. Carbohydrates in protein. 3 The preparation and some of the properties of a glycopeptide from hen's-egg albumin.
    Biochem J. 1961 Mar;78:518-27 PMID: 13790185
  12. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  13. Cell recognition in plants: Determinants of the stigma surface and their pollen interactions.
    Proc Natl Acad Sci U S A. 1976 Aug;73(8):2788-92 PMID: 16592342
  14. Cancer-associated serum galactosyltransferase activity. Demonstration in an animal model system.
    J Biol Chem. 1977 Mar 10;252(5):1807-13 PMID: 65353
  15. Selective inhibition of a plasma fucosyltransferase by N-ethylmaleimide.
    Biochem Biophys Res Commun. 1977 Feb 7;74(3):1001-6 PMID: 66052
  16. A comparison of membrane components of normal and transformed BALB/c cells.
    Biochemistry. 1977 Jun 28;16(13):2902-8 PMID: 195596
  17. Processing of high mannose oligosaccharides to form complex type oligosaccharides on the newly synthesized polypeptides of the vesicular stomatitis virus G protein and the IgG heavy chain.
    J Biol Chem. 1978 Feb 10;253(3):716-22 PMID: 202596
  18. Galactosyltransferase activities in human sera: detection of a cancer-associated isoenzyme.
    Biochem Biophys Res Commun. 1975 Jul 22;65(2):545-51 PMID: 238533
  19. Isolation of novel glycoprotein HNC1beta from the urine of a patient with acute monocytic leukemia.
    Cancer Res. 1978 Mar;38(3):602-7 PMID: 272227
  20. Increase in serum and tissue glycosyltransferases and glycosidases in tumor-bearing rats.
    Cancer Res. 1977 Sep;37(9):3442-7 PMID: 406991
  21. Alterations in serum glycosyltransferases and 5'-nucleotidase in breast cancer patients.
    Cancer Res. 1978 Mar;38(3):723-8 PMID: 626976
  22. A cancer-associated galactosyltransferase isoenzyme.
    N Engl J Med. 1978 Sep 28;299(13):703-5 PMID: 683253
  23. Role of cell membrane galactosyltransferase in concanavalin A agglutination of erythrocytes.
    Biochem J. 1975 Jan;146(1):213-21 PMID: 807201
  24. Transformation-dependent modifications in released and cell-bound surface proteins detected by antisera to shed antigens.
    Cancer Res. 1976 Oct;36(10):3568-73 PMID: 821604
  25. Concomitant elevations in serum sialytransferase activity and sialic acid content in rats with metastasizing mammary tumors.
    Science. 1977 Feb 11;195(4278):577-80 PMID: 835014
  26. Growth-dependent alterations in oligomannosyl cores of glycopeptides.
    Proc Natl Acad Sci U S A. 1975 Aug;72(8):3139-43 PMID: 1059100
  27. Cancer-associated isoenzyme of serum galactosyltransferase.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1319-22 PMID: 1063413
  28. Isolation of retina-specific cell-aggregating factor from membranes of embryonic neural retina tissue.
    Proc Natl Acad Sci U S A. 1976 Oct;73(10):3594-8 PMID: 1068472
  29. Carcinoembryonic antigen (CEA): molecular biology and clinical significance.
    Biochim Biophys Acta. 1975 Jul 11;417(2):123-52 PMID: 1096961
  30. An endotoxin-induced serum factor that causes necrosis of tumors.
    Proc Natl Acad Sci U S A. 1975 Sep;72(9):3666-70 PMID: 1103152
  31. Cell surface glycosyltransferases.
    Biochim Biophys Acta. 1975 Dec 29;415(4):473-512 PMID: 1106769
  32. Glycoprotein metabolism in inflammatory and neoplastic diseases of the human colon.
    Cancer Res. 1975 Aug;35(8):2092-7 PMID: 1149023
  33. The binding of tissue-specific adhesive molecules to the cell surface. A molecular basis for specificity.
    Biochemistry. 1975 Jan 14;14(1):167-71 PMID: 1167336
  34. Effect of tumour necrosis factor on cultured human melanoma cells.
    Nature. 1975 Dec 25;258(5537):731-2 PMID: 1207755
  35. Alterations of membrane glycopeptides in human colonic adenocarcinoma.
    Proc Natl Acad Sci U S A. 1974 Dec;71(12):4869-73 PMID: 4140512
  36. A molecular model for cell interactions.
    Q Rev Biol. 1973 Dec;48(4):541-63 PMID: 4273570
  37. A comparative study of glycoproteins from the surface of control and Rous sarcoma virus transformed hamster cells.
    Biochemistry. 1970 Nov 10;9(23):4567-76 PMID: 4319754
  38. Glycopeptides from the surface of control and virus-transformed cells.
    Science. 1971 Apr 9;172(3979):169-71 PMID: 4323250
  39. Surface glycoproteins of normal and transformed cells: a difference determined by sialic acid and a growth-dependent sialyl transferase.
    Proc Natl Acad Sci U S A. 1972 Jul;69(7):1838-42 PMID: 4340161
  40. Cell contact-dependent increase in membrane D-galactopyranosyl-like residues on normal, but not virus- or spontaneously-transformed, murine fibroblasts.
    Proc Natl Acad Sci U S A. 1973 Jun;70(6):1672-6 PMID: 4352648
  41. The inhibition of chick embryo cell growth by medium obtained from cultures of Rous sarcoma cells.
    Exp Cell Res. 1966 Jan;41(1):149-61 PMID: 4378142
  42. Cell contact dependence of surface galactosyltransferase activity as a function of the cell cycle.
    J Cell Biol. 1974 Dec;63(3):796-805 PMID: 4474177
  43. A factor from a transformed cell line that affects cell migration.
    Proc Natl Acad Sci U S A. 1973 Feb;70(2):369-72 PMID: 4510281
  44. Carcinoembryonic antigen. Quantitative variations in circulating levels in benign and malignant digestive tract diseases.
    Adv Intern Med. 1974;19:413-33 PMID: 4592719
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1780279
Published
1979-02-15
Pages
279-87
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1186513
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1780279 PMC Full Text View journal details
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