Surface glycoproteins of normal and transformed cells: a difference determined by sialic acid and a growth-dependent sialyl transferase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 7 ·1972-07-00 ·Pages 1838-42

Warren L, Fuhrer JP, Buck CA

Abstract

The pattern of elution from a column of Sephadex G-50 of a fucose-labeled carbohydrate component derived from the surface glycoproteins of transformed cells can be altered to resemble that from untransformed cells by enzymatic removal of the sialic acid. These results indicate that the consistent differences found between control and virus-transformed cells may depend upon a relatively specific sialyl transferase that is found in greater amounts (2.5- to 11-times) in transformed cells than in control cells, and in dividing cells as compared to nondividing cells.

MeSH Terms

Animals Avian Sarcoma Viruses Carbohydrates/isolation & purification Carbon Isotopes Cell Division Cell Line Cell Membrane/analysis Cell Transformation, Neoplastic Chromatography, Gel Cricetinae Fucose/metabolism Glycoproteins/analysis Kidney/cytology,enzymology,metabolism Neuraminic Acids Neuraminidase Nucleoside Diphosphate Sugars Polyomavirus Pronase Transferases/analysis Tritium

Chemicals

Carbohydrates Carbon Isotopes Glycoproteins Neuraminic Acids Nucleoside Diphosphate Sugars Tritium Fucose Transferases Neuraminidase Pronase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Warren L

Fuhrer J P

Buck C A

References (15)

15 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.7.1838

Published

1972-07-00

Pages

1838-42

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC426814

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.7.1838 PMC Full Text View journal details

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Surface glycoproteins of normal and transformed cells: a difference determined by sialic acid and a growth-dependent sialyl transferase.

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