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PMID: 8692842 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human cyclin-dependent kinase-activating kinase exists in three distinct complexes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 13 ·1996-06-25 ·Pages 6488-93

Drapkin R, Le Roy G, Cho H, Akoulitchev S, Reinberg D

Abstract

Transcription factor IIH (TFIIH) is a multisubunit complex required for transcription and for DNA nucleotide excision repair. TFIIH possesses three enzymatic activities: (i) an ATP-dependent DNA helicase, (ii) a DNA-dependent ATPase, and (iii) a kinase with specificity for the carboxyl-terminal domain of RNA polymerase II. The kinase activity was recently identified as the cdk (cyclin-dependent kinase) activating kinase, CAK, composed of cdk7, cyclin H, and MAT-1. Here we report the isolation and characterization of three distinct CAK-containing complexes from HeLa nuclear extracts: CAK, a novel CAK-ERCC2 complex, and TFIIH. CAK-ERCC2 can efficiently associate with core-TFIIH to reconstitute holo-TFIIH transcription activity. We present evidence proposing a critical role for ERCC2 in mediating the association of CAK with core TFIIH subunits.

MeSH Terms
Cyclin-Dependent Kinases/metabolism Enzyme Activation HeLa Cells Humans Isoenzymes/metabolism Transcription Factor TFIIH Transcription Factors/metabolism Transcription Factors, TFII
Chemicals
Isoenzymes Transcription Factors Transcription Factors, TFII Transcription Factor TFIIH Cyclin-Dependent Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Drapkin R
Howard Hughes Medical Institute, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, 088854-5635, USA.
Le Roy G
Cho H
Akoulitchev S
Reinberg D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.13.6488
Published
1996-06-25
Pages
6488-93
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC39050
Subset
IM
Grants
NIGMS NIH HHS · GM08360 · United States
NIGMS NIH HHS · GM47484 · United States
NIGMS NIH HHS · GM48518 · United States
External Links
PubMed source DOI: 10.1073/pnas.93.13.6488 PMC Full Text View journal details
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