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PMID: 8676457 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The hepatitis B virus posttranscriptional regulatory element is composed of two subelements.

Journal of virology ·Vol. 70 ·No. 7 ·1996-07-00 ·Pages 4345-51

Donello JE, Beeche AA, Smith GJ, Lucero GR, Hope TJ

Abstract

The RNAs of the hepatitis B virus (HBV) contain a cis-acting regulatory element which facilitates the cytoplasmic localization of unspliced transcripts (J. Huang and T. J. Liang, Mol. Cell. Biol. 13:7476-7486, 1993, and Z. M. Huang and T. S. Yen, J. Virl. 68:3193-3199, 1994). Such localization is presumed to be mediated by cellular factors which interact with the element. The HBV posttranscriptional regulatory element (HBVPRE) can efficiently activate an RNA export reporter system in an orientation-dependent and position-independent manner. Deletion analysis reveals that the HBVPRE consists of two subelements which function synergistically. A synergistic effect was also observed when the 5' (PREalpha) or 3' (PREbeta) subelements were duplicated. The bipartite structure of the HBVPRE is reminiscent of reports that the high-affinity binding sites of the Rev-like proteins must be duplicated to function efficiently (M. Grone, E. Hoffmann, S. Berchtold, B.R. Cullen, and R. Grassmann, Virology 204:144-152, 1994; X. Huang, T.J. Hope, B.L. Bond, D. McDonald, K. Grahl, and T. G. Parslow, J. Virol. 65:2131-2134, 1991; and D. McDonald, T. J. Hope, and T. G. Parslow, J. Virol. 66:7232-7238, 1992).

MeSH Terms
Animals Base Sequence Cell Line Cell Line, Transformed Chlorocebus aethiops Cytoplasm/metabolism,virology DNA Primers Exons Hepatitis B virus/genetics Humans Introns Molecular Sequence Data RNA Processing, Post-Transcriptional RNA Splicing RNA, Viral/genetics,metabolism Regulatory Sequences, Nucleic Acid Sequence Deletion
Chemicals
DNA Primers RNA, Viral
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Donello J E
Infectious Disease Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA.
Beeche A A
Smith G J
Lucero G R
Hope T J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.7.4345-4351.1996
Published
1996-07-00
Pages
4345-51
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC190367
Subset
IM
Databases
GENBANK
D00329, J02029, K03455
External Links
PubMed source DOI: 10.1128/JVI.70.7.4345-4351.1996 PMC Full Text View journal details
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