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PMID: 8670815 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The rpsO mRNA of Escherichia coli is polyadenylated at multiple sites resulting from endonucleolytic processing and exonucleolytic degradation.

The EMBO journal ·Vol. 15 ·No. 12 ·1996-06-17 ·Pages 3144-52

Haugel-Nielsen J, Hajnsdorf E, Regnier P

Abstract

The rpsO monocistronic messenger, encoding ribosomal protein S15, is destabilized upon polyadenylation occurring at the hairpin structure of the transcription terminator t1. We report that mRNA fragments differing from the monocistronic transcript by their 3' termini are also polyadenylated in the absence of polynucleotide phosphorylase and RNase II. Some of these 3' extremities result from endonucleolytic cleavages by RNase E and RNase III and from exonucleolytic degradation. Most of these mRNA fragments are destabilized upon polyadenylation with the exception of the RNA species generated by RNase III. RNase E appears to reduce the amount of poly(A) added at the transcription terminator t1.

MeSH Terms
Base Sequence Cell Nucleolus/enzymology Endoribonucleases/metabolism Escherichia coli/genetics Escherichia coli Proteins Exonucleases/metabolism Hydrolysis Molecular Sequence Data RNA Processing, Post-Transcriptional RNA, Messenger/genetics,metabolism Ribonuclease III Ribosomal Proteins/genetics
Chemicals
Escherichia coli Proteins RNA, Messenger Ribosomal Proteins ribosomal protein S15 Endoribonucleases Exonucleases Ribonuclease III ribonuclease III, E coli ribonuclease E
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Haugel-Nielsen J
Institut de Biologie Physico-Chimique, Paris, France.
Hajnsdorf E
Regnier P
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37 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-06-17
Pages
3144-52
Language
English
Region
England
NLM ID
8208664
PMCID
PMC450257
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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