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PMID: 2677604 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and characterization of an Escherichia coli gene, pcnB, affecting plasmid copy number.

Molecular microbiology ·Vol. 3 ·No. 7 ·1989-07-00 ·Pages 903-10

March JB, Colloms MD, Hart-Davis D, Oliver IR, Masters M

Abstract

A gene, pcnB, affecting the copy number of ColE1-related plasmids has been cloned and mapped to 3.6 min on the Escherichia coli chromosome between panD and fhu. The gene encodes a previously undescribed 48 kD protein. Several independently isolated mutants exhibiting the same phenotype, reduced copy number, have been shown to be pcnB-.

MeSH Terms

Alleles Ampicillin Resistance Bacterial Proteins/genetics Cloning, Molecular Escherichia coli/genetics Gene Expression Regulation Genes, Bacterial Molecular Weight Mutation Phenotype Plasmids Protein Biosynthesis Restriction Mapping Transcription, Genetic Transduction, Genetic

Chemicals

Bacterial Proteins

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

March J B

Department of Molecular Biology, University of Edinburgh.

Colloms M D

Hart-Davis D

Oliver I R

Masters M

Article Info

Journal

Molecular microbiology

Abbr.

Mol Microbiol

ISSN

0950-382X

DOI

10.1111/j.1365-2958.1989.tb00239.x

Published

1989-07-00

Pages

903-10

Language

English

Region

England

NLM ID

8712028

Subset

External Links

PubMed source DOI: 10.1111/j.1365-2958.1989.tb00239.x View journal details

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Cloning and characterization of an Escherichia coli gene, pcnB, affecting plasmid copy number.

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