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PMID: 8665864 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A splice hepadnavirus RNA that is essential for virus replication.

The EMBO journal ·Vol. 15 ·No. 10 ·1996-05-15 ·Pages 2565-74

Obert S, Zachmann-Brand B, Deindl E, Tucker W, Bartenschlager R, Schaller H

Abstract

According to the current model of hepadnavirus gene expression, the viral envelope proteins are produced from unspliced subgenomic RNAs, in contrast to the retroviral mechanism, where the subgenomic env RNA is generated by RNA splicing. We now describe and characterize a novel duck hepatitis B virus RNA species which is derived from the RNA pregenome by loss of a 1.15 kb intron. This RNA (termed spliced L RNA) codes for the large surface protein (L protein), as does the previously described unspliced mRNA (the preS RNA); however, it differs in 5' leader sequence and promoter control. Mutational analysis indicates that the spliced L RNA is functionally important for virus replication in infected hepatocytes and ducks, but not for virus formation from transfected DNA genomes. This suggests that the newly discovered second pathway for L protein synthesis plays a distinct role in an early step in the viral life cycle.

MeSH Terms
Animals Base Sequence Ducks/virology Hepadnaviridae Infections/virology Hepatitis B Virus, Duck/genetics,physiology Introns/genetics Liver/virology Molecular Sequence Data Polymerase Chain Reaction RNA Splicing/physiology RNA, Viral/physiology Sequence Alignment Sequence Homology, Nucleic Acid Transfection Viral Envelope Proteins/genetics Virus Replication/physiology
Chemicals
L protein, hepatitis B virus RNA, Viral Viral Envelope Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Obert S
Zentrum für Molekulare Biologie Heidelberg (ZMBH), Universität Heidelberg, Germany.
Zachmann-Brand B
Deindl E
Tucker W
Bartenschlager R
Schaller H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-05-15
Pages
2565-74
Language
English
Region
England
NLM ID
8208664
PMCID
PMC450189
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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