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PMID: 8657139 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transcriptional corepression in vitro: a Mot1p-associated form of TATA-binding protein is required for repression by Leu3p.

Molecular and cellular biology ·Vol. 16 ·No. 4 ·1996-04-00 ·Pages 1641-8

Wade PA, Jaehning JA

Abstract

Signals from transcriptional activators to the general mRNA transcription apparatus are communicated by factors associated with RNA polymerase II or the TATA-binding protein (TBP). Currently, little is known about how gene-specific transcription repressors communicate with RNA polymerase II. We have analyzed the requirements for repression by the saccharomyces cerevisiae Leu3 protein (Leu3p) in a reconstituted transcription system. We have identified a complex form of TBP which is required for communication of the repressing signal. This TFIID-like complex contains a known TBP-associated protein, Mot1p, which has been implicated in the repression of a subset of yeast genes by genetic analysis. Leu3p-dependent repression can be reconstituted with purified Mot1p and recombinant TBP. In addition, a mutation in the Mot1 gene leads to partial derepression of the Leu3p-dependent LEU2 promoter. These in vivo and in vitro observations define a role for Mot1p as a transcriptional corepressor.

MeSH Terms
Adenosine Triphosphatases DNA Helicases/genetics,metabolism DNA-Binding Proteins/genetics,metabolism Fungal Proteins/genetics,metabolism Mutation Recombinant Proteins/genetics,metabolism Repressor Proteins/genetics,metabolism Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins TATA Box TATA-Binding Protein Associated Factors TATA-Box Binding Protein Trans-Activators/genetics,metabolism Transcription Factors/genetics,metabolism Transcription, Genetic
Chemicals
DNA-Binding Proteins Fungal Proteins LEU3 protein, S cerevisiae Recombinant Proteins Repressor Proteins Saccharomyces cerevisiae Proteins TATA-Binding Protein Associated Factors TATA-Box Binding Protein Trans-Activators Transcription Factors Adenosine Triphosphatases MOT1 protein, S cerevisiae DNA Helicases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wade P A
Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, Denver, 80262, USA.
Jaehning J A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.4.1641
Published
1996-04-00
Pages
1641-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231150
Subset
IM
Grants
NIGMS NIH HHS · R01 GM 38101 · United States
External Links
PubMed source DOI: 10.1128/MCB.16.4.1641 PMC Full Text View journal details
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