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PMID: 8655533 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

redD and actII-ORF4, pathway-specific regulatory genes for antibiotic production in Streptomyces coelicolor A3(2), are transcribed in vitro by an RNA polymerase holoenzyme containing sigma hrdD.

Journal of bacteriology ·Vol. 178 ·No. 11 ·1996-06-00 ·Pages 3402-5

Fujii T, Gramajo HC, Takano E, Bibb MJ

Abstract

redD and actII-ORF4, regulatory genes required for synthesis of the antibiotics undecylprodigiosin and actinorhodin by Streptomyces coelicolor A3(2), were transcribed in vitro by an RNA polymerase holoenzyme containing sigma hrdD. Disruption of hrdD had no effect on antibiotic production, indicating that redD and actII-ORF4 are transcribed in vivo by at least one other RNA polymerase holoenzyme. These data provide the first experimental evidence that HrdD can function as a sigma factor.

MeSH Terms
Amino Acid Sequence Anthraquinones/metabolism Anti-Bacterial Agents/biosynthesis DNA-Directed RNA Polymerases/pharmacology Genes, Bacterial Genes, Regulator Molecular Sequence Data Open Reading Frames Prodigiosin/analogs & derivatives,biosynthesis Sigma Factor/physiology Streptomyces/genetics,metabolism Transcription, Genetic
Chemicals
Anthraquinones Anti-Bacterial Agents Sigma Factor undecylprodigiosin DNA-Directed RNA Polymerases actinorhodin Prodigiosin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fujii T
Department of Genetics, Jonn Innes Centre, Norwich Research Park, Colney, United Kingdom.
Gramajo H C
Takano E
Bibb M J
References (24)
24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.11.3402-3405.1996
Published
1996-06-00
Pages
3402-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC178105
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.178.11.3402-3405.1996 PMC Full Text View journal details
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