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PMID: 8648695 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Complete nucleotide sequence and transcriptional analysis of snakehead fish retrovirus.

Journal of virology ·Vol. 70 ·No. 6 ·1996-06-00 ·Pages 3606-16

Hart D, Frerichs GN, Rambaut A, Onions DE

Abstract

The complete genome of the snakehead fish retrovirus has been cloned and sequenced, and its transcriptional profile in cell culture has been determined. The 11.2-kb provirus displays a complex expression pattern capable of encoding accessory proteins and is unique in the predicted location of the env initiation codon and signal peptide upstream of gag and the common splice donor site. The virus is distinguishable from all known retrovirus groups by the presence of an arginine tRNA primer binding site. The coding regions are highly divergent and show a number of unusual characteristics, including a large Gag coiled-coil region, a Pol domain of unknown function, and a long, lentiviral-like, Env cytoplasmic domain. Phylogenetic analysis of the Pol sequence emphasizes the divergent nature of the virus from the avian and mammalian retroviruses. The snakehead virus is also distinct from a previously characterized complex fish retrovirus, suggesting that discrete groups of these viruses have yet to be identified in the lower vertebrates.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Fishes/virology Genes, env Genes, gag Genes, pol Molecular Sequence Data Open Reading Frames Phylogeny RNA, Viral/chemistry Repetitive Sequences, Nucleic Acid Retroviridae/classification,genetics Transcription, Genetic
Chemicals
RNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hart D
Department of Veterinary Pathology, University of Glasgow Veterinary School, United Kingdom.
Frerichs G N
Rambaut A
Onions D E
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.6.3606-3616.1996
Published
1996-06-00
Pages
3606-16
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC190236
Subset
IM
Databases
GENBANK
U26458
External Links
PubMed source DOI: 10.1128/JVI.70.6.3606-3616.1996 PMC Full Text View journal details
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