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PMID: 8636236 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cell type-specific roles for Cdc42, Rac, and RhoL in Drosophila oogenesis.

The Journal of cell biology ·Vol. 133 ·No. 3 ·1996-05-00 ·Pages 617-30

Murphy AM, Montell DJ

Abstract

The Rho subfamily of GTPases has been shown to regulate cellular morphology. We report the discovery of a new member of the Rho family, named RhoL, which is equally similar to Rac, Rho, and Cdc42. Expression of a dominant-negative RhoL transgene in the Drosophila ovary caused nurse cells to collapse and fuse together. Mutant forms of Cdc42 mimicked this effect. Expression of constitutively active RhoL led to nurse cell subcortical actin breakdown and disruption of nurse cell-follicle cell contacts, followed by germ cell apoptosis. In contrast, Rac activity was specifically required for migration of a subset of follicle cells called border cells. All three activities were necessary for normal transfer of nurse cell cytoplasm to the oocyte. These results suggest that Rho protein activities have cell type-specific effects on morphogenesis.

MeSH Terms
Actins/metabolism Animals Base Sequence Biological Transport/physiology Cell Movement/physiology Cloning, Molecular Cytoplasm/metabolism Drosophila/cytology Drosophila Proteins Female GTP Phosphohydrolases/metabolism,physiology GTP-Binding Proteins/physiology Germ Cells/cytology,enzymology Insect Hormones/physiology Membrane Proteins/physiology Molecular Sequence Data Oogenesis/physiology Ovum/cytology,enzymology Sequence Homology, Amino Acid rac GTP-Binding Proteins
Chemicals
Actins Cdc42 protein, Drosophila Drosophila Proteins Insect Hormones Membrane Proteins Rac1 protein, Drosophila Rho protein, Drosophila Stet protein, Drosophila GTP Phosphohydrolases GTP-Binding Proteins rac GTP-Binding Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Murphy A M
Department of Biological Chemistry, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205-2185, USA.
Montell D J
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.133.3.617
Published
1996-05-00
Pages
617-30
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120826
Subset
IM
Grants
PHS HHS · R296M46425 · United States
Databases
GENBANK
U50314
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1083/jcb.133.3.617 PMC Full Text View journal details
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