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PMID: 8635467 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Escherichia coli protein analogs StpA and H-NS: regulatory loops, similar and disparate effects on nucleic acid dynamics.

The EMBO journal ·Vol. 15 ·No. 6 ·1996-03-15 ·Pages 1340-9

Zhang A, Rimsky S, Reaban ME, Buc H, Belfort M

Abstract

Expression of the Escherichia coli StpA protein was investigated and a functional comparison undertaken with the structurally analogous nucleoid protein H-NS. Analysis of stpA and hns expression indicated that although stpA transcript levels are much lower than those of hns, the two gene products are capable of both negative autogenous control and cross-regulation. Examination of cellular proteins in stpA, hns, or stpA-hns backgrounds revealed that StpA can repress and activate a subset of H-NS-regulated genes. Mechanistic parallels in regulation of gene expression are indicated by the ability of both proteins to inhibit transcription from promoters containing curved DNA sequences, and to form nucleoprotein structures that constrain DNA supercoils. Despite their functional similarities, each molecule is capable of independent activities. Thus, H-NS regulates a class of genes that are unaffected by StpA in vivo, whereas StpA has much stronger RNA chaperone activity in vitro. We therefore propose that in addition to its role as a molecular back-up of H-NS, StpA's superior effect on RNA may be exploited under some specific cellular conditions to promote differential gene expression.

MeSH Terms
Bacterial Outer Membrane Proteins/genetics,metabolism Bacterial Proteins/genetics,metabolism Base Sequence DNA, Bacterial/metabolism DNA-Binding Proteins/genetics,metabolism Escherichia coli/genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial Models, Genetic Molecular Chaperones Molecular Sequence Data Nucleic Acid Conformation RNA Splicing RNA, Bacterial/metabolism RNA-Binding Proteins/metabolism Transcription, Genetic
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins DNA, Bacterial DNA-Binding Proteins Escherichia coli Proteins H-NS protein, bacteria Molecular Chaperones RNA, Bacterial RNA-Binding Proteins StpA protein, E coli
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Zhang A
Molecular Genetics Program, New York State Department of Health and School of Public Health, Albany 12201-2002, USA.
Rimsky S
Reaban M E
Buc H
Belfort M
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44 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-03-15
Pages
1340-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC450038
Subset
IM
Grants
NIGMS NIH HHS · GM14915 · United States
NIGMS NIH HHS · GM39422 · United States
NIGMS NIH HHS · GM44844 · United States
External Links
PubMed source PMC Full Text View journal details
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