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PMID: 7534408 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

A small RNA acts as an antisilencer of the H-NS-silenced rcsA gene of Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 6 ·1995-03-14 ·Pages 2003-7

Sledjeski D, Gottesman S

Abstract

The regulation of capsular polysaccharide synthesis in Escherichia coli K-12 depends on the level of an unstable positive regulator, RcsA. The amount of RcsA protein is limited both by its rapid degradation by Lon, an ATP-dependent protease, and by its low level of synthesis. We have found that the low level of expression from the rcsA promoter is due to transcriptional silencing by the histone-like protein H-NS; this silencing is sensitive to both sequence and context in a region upstream of the -35 region of the promoter. A small (85-nt) RNA, DsrA, when overproduced, activates transcription of rcsA::lacZ fusions by counteracting H-NS silencing. DsrA RNA does not show any extended homology with the rcsA promoter or other sequenced regions of E. coli. Since the stimulation of rcsA transcription by this small RNA does not depend on any sequences from within the rcsA transcript, DsrA acts, either directly or indirectly, on rcsA transcription initiation.

MeSH Terms
ATP-Dependent Proteases Adenosine Triphosphatases/metabolism Bacterial Outer Membrane Proteins/metabolism Bacterial Proteins/biosynthesis,genetics Base Sequence Cloning, Molecular DNA Primers DNA-Binding Proteins/metabolism Escherichia coli/genetics,metabolism Escherichia coli Proteins Gene Expression Genes, Bacterial Heat-Shock Proteins/metabolism Klebsiella pneumoniae/genetics Molecular Sequence Data Nucleic Acid Conformation Point Mutation Protease La RNA Caps/metabolism RNA, Bacterial/metabolism Recombinant Fusion Proteins/biosynthesis Restriction Mapping Serine Endopeptidases/metabolism Transcription, Genetic beta-Galactosidase/biosynthesis
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins DNA Primers DNA-Binding Proteins Escherichia coli Proteins H-NS protein, bacteria Heat-Shock Proteins RNA Caps RNA, Bacterial Recombinant Fusion Proteins RcsA protein, E coli beta-Galactosidase ATP-Dependent Proteases Serine Endopeptidases Lon protein, E coli Protease La Adenosine Triphosphatases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sledjeski D
Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4255.
Gottesman S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.6.2003
Published
1995-03-14
Pages
2003-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC42411
Subset
IM
Grants
NIGMS NIH HHS · F32GM14776-01 · United States
Databases
GENBANK
M58003, U17136, U17137
External Links
PubMed source DOI: 10.1073/pnas.92.6.2003 PMC Full Text View journal details
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