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PMID: 8633867 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The genes for secretion and maturation of lactococcins are located on the chromosome of Lactococcus lactis IL1403.

Applied and environmental microbiology ·Vol. 62 ·No. 5 ·1996-05-00 ·Pages 1689-92

Venema K, Dost MH, Beun PA, Haandrikman AJ, Venema G, Kok J

Abstract

Southern hybridization and PCR analysis were used to show that Lactococcus lactis IL1403, a plasmid-free strain that does not produce bacteriocin, contains genes on its chromosome that are highly homologous to lcnC and lcnD and encode the lactococcin secretion and maturation system. The lcnC and lcnD homologs on the chromosome of IL1403 were interrupted independently by Campbell-type integrations. Both insertion mutants were unable to secrete active lactococcin. Part of the chromosomal lcnC gene was cloned and sequenced. Only a few nucleotide substitutions occurred, compared with the plasmid-encoded lcnC gene, and these did not lead to changes in the deduced amino acid sequence. No genes homologous to those for lactococcin A, B, or M could be detected in IL1403, and the strain does not produce bacteriocin activity.

MeSH Terms
Amino Acid Sequence Bacteriocins/genetics Base Sequence Chromosome Mapping Cloning, Molecular DNA, Bacterial/genetics Lactococcus lactis/genetics,metabolism Molecular Sequence Data
Chemicals
Bacteriocins DNA, Bacterial lactococcin A
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Venema K
Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands.
Dost M H
Beun P A
Haandrikman A J
Venema G
Kok J
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.62.5.1689-1692.1996
Published
1996-05-00
Pages
1689-92
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC167943
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.62.5.1689-1692.1996 PMC Full Text View journal details
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