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PMID: 8631685 Published · ppublish English Journal Article

maoB, a gene that encodes a positive regulator of the monoamine oxidase gene (maoA) in Escherichia coli.

Journal of bacteriology ·Vol. 178 ·No. 10 ·1996-05-00 ·Pages 2941-7

Yamashita M, Azakami H, Yokoro N, Roh JH, Suzuki H, Kumagai H, Murooka Y

Abstract

The structural gene for copper- and topa quinone-containing monoamine oxidase (maoA) and an unknown amine oxidase gene have been located at 30.9 min on the Escherichia coli chromosome. Deletion analysis showed that the unknown gene was located within a 1.1-kb cloned fragment adjacent to the maoA gene. The nucleotide sequence of this fragment was determined, and a single open reading frame (maoB) consisting of 903 bp was found. The gene encoded a polypeptide with a predicted molecular mass of 34,619 Da which was correlated with the migration on a sodium dodecyl sulfate-polyacrylamide gel. The predicted amino acid sequence of the MaoB protein was identical to the NH2-terminal amino acid sequence derived by Edman degradation of the protein synthesized under the self-promoter. No homology of the nucleotide sequence of maoB to the sequences of any reported genes was found. However, the amino acid sequence of MaoB showed a high level of homology with respect to the helix-turn-helix motif of the AraC family in its C terminus. The homology search and disruption of maoA on the chromosome led to the conclusion that MaoB is a transcriptional activator of maoA but not an amine oxidase. The consensus sequence of the cyclic AMP-cyclic AMP receptor protein complex binding domain was adjacent to the putative promoter for the maoB gene. By use of lac gene fusions with the maoA and maoB genes, we showed that the maoA gene is regulated by tyramine and MaoB and that the expression of the maoB gene is subject to catabolite repression. Thus, it seems likely that tyramine and the MaoB protein activate the transcription of maoA by binding to the regulatory region of the maoA gene.

MeSH Terms
Amino Acid Sequence Carrier Proteins Cloning, Molecular Cyclic AMP/metabolism Cyclic AMP Receptor Protein/metabolism DNA Mutational Analysis Escherichia coli/genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial Gene Expression Regulation, Developmental Genes, Bacterial Genes, Regulator Molecular Sequence Data Monoamine Oxidase/biosynthesis Mutagenesis Sequence Analysis, DNA Sequence Deletion Sequence Homology Trans-Activators/genetics Transcription, Genetic
Chemicals
Carrier Proteins Cyclic AMP Receptor Protein Escherichia coli Proteins MaoB protein, E coli Trans-Activators Cyclic AMP Monoamine Oxidase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Yamashita M
Department of Biotechnology, Graduate School of Engineering, Osaka University, Japan.
Azakami H
Yokoro N
Roh J H
Suzuki H
Kumagai H
Murooka Y
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.10.2941-2947.1996
Published
1996-05-00
Pages
2941-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC178032
Subset
IM
Databases
GENBANK
D67041
External Links
PubMed source DOI: 10.1128/jb.178.10.2941-2947.1996 PMC Full Text View journal details
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