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PMID: 8622991 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Human TOP3: a single-copy gene encoding DNA topoisomerase III.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 8 ·1996-04-16 ·Pages 3653-7

Hanai R, Caron PR, Wang JC

Abstract

A human cDNA encoding a protein homologous to the Escherichia coli DNA topoisomerase I subfamily of enzymes has been identified through cloning and sequencing. Expressing the cloned human cDNA in yeast (delta)top1 cells lacking endogenous DNA topoisomerase I yielded an activity in cell extracts that specifically reduces the number of supercoils in a highly negatively supercoiled DNA. On the basis of these results, the human gene containing the cDNA sequence has been denoted TOP3, and the protein it encodes has been denoted DNA topoisomerase III. Screening of a panel of human-rodent somatic hybrids and fluorescence in situ hybridization of cloned TOP3 genomic DNA to metaphase chromosomes indicate that human TOP3 is a single-copy gene located at chromosome 17p11.2-12.

MeSH Terms
Animals Chromosome Mapping Chromosomes, Human, Pair 17/genetics Cloning, Molecular Cricetinae DNA Topoisomerases, Type I/genetics,metabolism DNA, Complementary/genetics DNA, Superhelical/metabolism Escherichia coli/enzymology,genetics Genes Humans Hybrid Cells In Vitro Techniques Mice Molecular Sequence Data Recombinant Proteins/genetics,metabolism Saccharomyces cerevisiae/genetics Sequence Homology, Amino Acid
Chemicals
DNA, Complementary DNA, Superhelical Recombinant Proteins DNA Topoisomerases, Type I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hanai R
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02139, USA.
Caron P R
Wang J C
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.8.3653
Published
1996-04-16
Pages
3653-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC39666
Subset
IM
Grants
NIGMS NIH HHS · GM24544 · United States
Databases
GENBANK
U43431
External Links
PubMed source DOI: 10.1073/pnas.93.8.3653 PMC Full Text View journal details
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