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PMID: 8393572 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNA topoisomerase I is essential in Drosophila melanogaster.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 14 ·1993-07-15 ·Pages 6656-60

Lee MP, Brown SD, Chen A, Hsieh TS

Abstract

Both biochemical and genetic experiments suggest that the type I DNA topoisomerase may participate in DNA replication, recombination, transcription, and other aspects of DNA metabolism. Despite its apparent importance, genetic studies in unicellular organisms including eubacteria and yeasts indicate that topoisomerase I is not essential for viability. We have previously isolated the cDNA clone encoding DNA topoisomerase I from Drosophila melanogaster. We report here the cytogenetic mapping of top1 to the X chromosome at 13C1 and isolation of top1 genomic DNA. Using P-element mutagenesis, we have isolated a mutant deficient in Drosophila topoisomerase I functions. Genetic studies of this mutant show that topoisomerase I is essential for the growth and development of the fruit fly, a multicellular organism. The biological functions of topoisomerase I are inferred from our analysis of the regulation of topoisomerase I expression during Drosophila development.

Related Genes
top1
MeSH Terms
Animals Chromosome Mapping Cloning, Molecular Crosses, Genetic DNA Topoisomerases, Type I/biosynthesis,genetics Drosophila melanogaster/enzymology,genetics,growth & development Genes, Insect/genetics Genes, Lethal/genetics In Situ Hybridization Mutagenesis, Insertional Mutation Sequence Analysis, DNA Sex Characteristics Transcription, Genetic X Chromosome
Chemicals
DNA Topoisomerases, Type I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lee M P
Department of Biochemistry, Duke University Medical Center, Durham, NC 27710.
Brown S D
Chen A
Hsieh T S
References (46)
46 references, click to expand
  1. An activity from mammalian cells that untwists superhelical DNA--a possible swivel for DNA replication (polyoma-ethidium bromide-mouse-embryo cells-dye binding assay).
    Proc Natl Acad Sci U S A. 1972 Jan;69(1):143-6 PMID: 4333036
  2. Cloning of yeast TOP1, the gene encoding DNA topoisomerase I, and construction of mutants defective in both DNA topoisomerase I and DNA topoisomerase II.
    Proc Natl Acad Sci U S A. 1985 Nov;82(21):7178-82 PMID: 2997777
  3. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  4. Changing rates of DNA and RNA synthesis in Drosophila embryos.
    Dev Biol. 1981 Feb;82(1):127-38 PMID: 6164584
  5. Mutations in the gene coding for Escherichia coli DNA topoisomerase I affect transcription and transposition.
    Proc Natl Acad Sci U S A. 1981 May;78(5):2747-51 PMID: 6265907
  6. Topoisomerase I interacts with transcribed regions in Drosophila cells.
    Cell. 1986 Feb 14;44(3):401-7 PMID: 3002635
  7. Parameters controlling transcriptional activation during early Drosophila development.
    Cell. 1986 Mar 28;44(6):871-7 PMID: 2420468
  8. Isolation and characterization of the gene encoding Drosophila DNA topoisomerase II.
    Proc Natl Acad Sci U S A. 1986 Jun;83(11):3664-8 PMID: 3012525
  9. X-linked female-sterile loci in Drosophila melanogaster.
    Genetics. 1986 Jul;113(3):695-712 PMID: 3089870
  10. Roles of DNA topoisomerases in simian virus 40 DNA replication in vitro.
    Proc Natl Acad Sci U S A. 1987 Feb;84(4):950-4 PMID: 3029765
  11. Need for DNA topoisomerase activity as a swivel for DNA replication for transcription of ribosomal RNA.
    Nature. 1987 Mar 26-Apr 1;326(6111):414-6 PMID: 2436053
  12. Expression of the Drosophila type II topoisomerase is developmentally regulated.
    Biochemistry. 1988 Jan 26;27(2):560-5 PMID: 2831969
  13. A stable genomic source of P element transposase in Drosophila melanogaster.
    Genetics. 1988 Mar;118(3):461-70 PMID: 2835286
  14. Mitotic recombination in the rDNA of S. cerevisiae is suppressed by the combined action of DNA topoisomerases I and II.
    Cell. 1988 Nov 4;55(3):413-25 PMID: 2902925
  15. Gene expression in regenerating liver in relation to cell proliferation and stress.
    Eur J Biochem. 1989 Mar 1;180(1):49-53 PMID: 2540002
  16. A subthreshold level of DNA topoisomerases leads to the excision of yeast rDNA as extrachromosomal rings.
    Cell. 1989 Jun 16;57(6):975-85 PMID: 2544296
  17. A hyper-recombination mutation in S. cerevisiae identifies a novel eukaryotic topoisomerase.
    Cell. 1989 Jul 28;58(2):409-19 PMID: 2546682
  18. Phorbol ester transiently increases topoisomerase I mRNA levels in human skin fibroblasts.
    J Biol Chem. 1989 Sep 5;264(25):14923-6 PMID: 2549041
  19. Function of DNA topoisomerases as replication swivels in Saccharomyces cerevisiae.
    J Mol Biol. 1989 Jul 20;208(2):257-67 PMID: 2549254
  20. Molecular cloning and DNA sequence analysis of Escherichia coli topB, the gene encoding topoisomerase III.
    J Biol Chem. 1989 Oct 25;264(30):17924-30 PMID: 2553698
  21. Expression of the type I DNA topoisomerase gene in adenovirus-5 infected human cells.
    Nucleic Acids Res. 1990 Feb 25;18(4):801-8 PMID: 2156232
  22. Expression of the topoisomerase I gene in serum stimulated human fibroblasts.
    Biochim Biophys Acta. 1990 Apr 6;1048(2-3):274-80 PMID: 2157495
  23. Structure and expression during development of Drosophila melanogaster gene for DNA polymerase alpha.
    Nucleic Acids Res. 1991 Sep 25;19(18):4991-8 PMID: 1923767
  24. A general topoisomerase I-dependent transcriptional repression in the stationary phase in yeast.
    Genes Dev. 1991 Dec;5(12A):2315-26 PMID: 1660829
  25. Creative blocks: cell-cycle checkpoints and feedback controls.
    Nature. 1992 Oct 15;359(6396):599-604 PMID: 1406993
  26. Isolation and characterization of a gene encoding DNA topoisomerase I in Drosophila melanogaster.
    Nucleic Acids Res. 1992 Dec 11;20(23):6177-82 PMID: 1335568
  27. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  28. Interaction between DNA and an Escherichia coli protein omega.
    J Mol Biol. 1971 Feb 14;55(3):523-33 PMID: 4927945
  29. The effect of heterozygous inversions on primary nondisjunction in Drosophila melanogaster.
    Genetics. 1962 Oct;47:1301-11 PMID: 13958677
  30. Transposition of cloned P elements into Drosophila germ line chromosomes.
    Science. 1982 Oct 22;218(4570):341-7 PMID: 6289435
  31. Genetic transformation of Drosophila with transposable element vectors.
    Science. 1982 Oct 22;218(4570):348-53 PMID: 6289436
  32. Escherichia coli DNA topoisomerase I mutants: increased supercoiling is corrected by mutations near gyrase genes.
    Cell. 1982 Nov;31(1):35-42 PMID: 6297751
  33. Escherichia coli DNA topoisomerase I mutants have compensatory mutations in DNA gyrase genes.
    Cell. 1982 Nov;31(1):43-51 PMID: 6297752
  34. DNA topoisomerases--enzymes that catalyse the breaking and rejoining of DNA.
    CRC Crit Rev Biochem. 1983;15(1):1-24 PMID: 6319080
  35. DNA topoisomerase II mutant of Saccharomyces cerevisiae: topoisomerase II is required for segregation of daughter molecules at the termination of DNA replication.
    Proc Natl Acad Sci U S A. 1984 May;81(9):2616-20 PMID: 6326134
  36. Localization of a cis-acting element responsible for the developmentally regulated amplification of Drosophila chorion genes.
    Cell. 1984 Aug;38(1):45-54 PMID: 6088075
  37. Isolation of type I and II DNA topoisomerase mutants from fission yeast: single and double mutants show different phenotypes in cell growth and chromatin organization.
    EMBO J. 1984 Aug;3(8):1737-44 PMID: 6090122
  38. Drosophila DNA topoisomerase I is associated with transcriptionally active regions of the genome.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):6958-62 PMID: 6095263
  39. Dysgenesis-induced instability of rosy locus transformation in Drosophila melanogaster: analysis of excision events and the selective recovery of control element deletions.
    Genetics. 1985 Jan;109(1):95-117 PMID: 2981758
  40. DNA topoisomerase II is required at the time of mitosis in yeast.
    Cell. 1985 Jun;41(2):553-63 PMID: 2985283
  41. Characterization and developmental expression of a Drosophila ras oncogene.
    Mol Cell Biol. 1985 Apr;5(4):885-9 PMID: 3921827
  42. DNA topoisomerases: enzymes that control DNA conformation.
    Curr Top Microbiol Immunol. 1985;114:19-102 PMID: 2986908
  43. Genetic analysis of mutations that compensate for loss of Escherichia coli DNA topoisomerase I.
    J Bacteriol. 1985 Jun;162(3):1173-9 PMID: 2987184
  44. Cloning, characterization, and sequence of the yeast DNA topoisomerase I gene.
    Proc Natl Acad Sci U S A. 1985 Jul;82(13):4374-8 PMID: 2989818
  45. DNA topoisomerases.
    Annu Rev Biochem. 1985;54:665-97 PMID: 2992360
  46. Nucleic acid hybridization to the DNA of cytological preparations.
    Methods Cell Biol. 1975;10:1-16 PMID: 1102840
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.14.6656
Published
1993-07-15
Pages
6656-60
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46991
Subset
IM
Grants
NIGMS NIH HHS · F31-GM14981 · United States
NIGMS NIH HHS · GM 29006 · United States
External Links
PubMed source DOI: 10.1073/pnas.90.14.6656 PMC Full Text View journal details
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