Home LiteratureArticle Details
PMID: 862233 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

A radiomicroassay for cytotoxic antibody to human spermatozoa. Quantification by tritiated actinomycin d.

Clinical and experimental immunology ·Vol. 27 ·No. 3 ·1977-03-00 ·Pages 469-77

Sung JS, Shizuya H, Black DD, Mumford DM

Abstract

A radiomicroassay for titration of spermocytotoxic antibody is described. The assay used [3H]AACTINOMYCIN D ([3H]Act D) to label damaged spermatozoa in a fashion analogous to penetration by vital dye. Optimal conditions for and some kinetics of the assay are presented. The assay is sensitive, reliable, simple to perform and uses only small amounts of serum and spermatozoa. Applied to sperm antibody positive human postvasectomy sera, the assay compared favourably in sensitivity eith vital dye microscopic observations and with parallel titration by the Isojima's immobilization tests.

MeSH Terms
Antibodies/analysis Cytotoxicity Tests, Immunologic/methods Dactinomycin Humans Male Spermatozoa/immunology Temperature Vasectomy
Chemicals
Antibodies Dactinomycin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sung J S
Shizuya H
Black D D
Mumford D M
References (9)
9 references, click to expand
  1. THE COMPLEMENT FIXATION TEST IN VIRUS ANTIBODY STUDIES.
    Can J Med Technol. 1964 Apr;26:35-41 PMID: 14137476
  2. Stereochemistry of actinomycin binding to DNA. II. Detailed molecular model of actinomycin-DNA complex and its implications.
    J Mol Biol. 1972 Jul 14;68(1):21-34 PMID: 4115109
  3. Sperm immunology, infertility, and fertility control.
    Obstet Gynecol. 1974 Oct;44(4):607-23 PMID: 4137940
  4. The ultrastructural lesions induced by antibody and complement in rabbit spermatozoa.
    Biol Reprod. 1974 Apr;10(3):293-308 PMID: 4462825
  5. Immunologic implications of vasectomy. II. Serum-mediated immunity.
    Fertil Steril. 1974 Jun;25(6):515-20 PMID: 4835607
  6. A simple and versatile harvesting device for processing radioactive label incorporated into and-or released from cells in microculture.
    J Immunol Methods. 1974 Jan;3(1):11-6 PMID: 4822884
  7. Microtechnique for simultaneous determination of immobilizing and cytotoxic sperm antibodies. Methodological and clinical studies.
    Clin Exp Immunol. 1975 Nov;22(2):256-64 PMID: 765021
  8. Antibody binding of spermatozoa.
    Fertil Steril. 1969 Jan-Feb;20(1):43-9 PMID: 5812531
  9. Interrelation between high levels of sperm antibodies, reduced penetration of cervical mucus by spermatozoa, and sterility in men.
    Acta Obstet Gynecol Scand. 1968;47(1):102-18 PMID: 5669830
Article Info
Journal
Clinical and experimental immunology
Abbr.
Clin Exp Immunol
ISSN
0009-9104
Published
1977-03-00
Pages
469-77
Language
English
Region
England
NLM ID
0057202
PMCID
PMC1540921
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2