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PMID: 8617234 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

MAT1, cdk7 and cyclin H form a kinase complex which is UV light-sensitive upon association with TFIIH.

The EMBO journal ·Vol. 15 ·No. 8 ·1996-04-15 ·Pages 1877-84

Adamczewski JP, Rossignol M, Tassan JP, Nigg EA, Moncollin V, Egly JM

Abstract

MAT1, cyclin H and cdk7 are part of TFIIH, a class II transcription factor which possesses numerous subunits of which several have been shown to be involved in processes other than transcription. Two of them, XPD (ERCC2) and XPB (ERCC3), are helicases involved in nucleotide excision repair (NER), whereas cdk7, cyclin H and MAT1 are thought to participate in cell cycle regulation. MAT1, cyclin H and cdk7 exist as a ternary complex either free or associated with TFIIH from which the latter can be dissociated at high salt concentration. MAT1 is strongly associated with cdk7 and cyclin H. Although not strictly required for the formation and activity of the complex, it stimulates its kinase activity. The kinase activity of TFIIH, which is constant during the cell cycle, is reduced after UV light irradiation.

MeSH Terms
Amino Acid Sequence Animals Cell Cycle Cell Line Cyclin H Cyclin-Dependent Kinases Cyclins/chemistry,genetics,radiation effects DNA Damage DNA Helicases/chemistry,genetics,radiation effects DNA Repair Macromolecular Substances Molecular Sequence Data Molecular Structure Multienzyme Complexes/chemistry,genetics,radiation effects Neoplasm Proteins/chemistry,genetics,radiation effects Protein Kinases/chemistry,genetics,radiation effects Protein Serine-Threonine Kinases/chemistry,genetics,radiation effects Recombinant Proteins/chemistry,genetics,radiation effects Transcription Factor TFIIH Transcription Factors/chemistry,genetics,radiation effects Transcription Factors, TFII Ultraviolet Rays
Chemicals
Cyclin H Cyclins Macromolecular Substances Multienzyme Complexes Neoplasm Proteins Recombinant Proteins Transcription Factors Transcription Factors, TFII Transcription Factor TFIIH Protein Kinases carboxy-terminal domain kinase Protein Serine-Threonine Kinases Cyclin-Dependent Kinases cyclin-dependent kinase-activating kinase DNA Helicases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Adamczewski J P
Institut de Génétique et de Biologie Moléculaire et Cellulaire, UPR 6520 (CNRS), Unité 184 (INSERM), CU de Strasbourg, France.
Rossignol M
Tassan J P
Nigg E A
Moncollin V
Egly J M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-04-15
Pages
1877-84
Language
English
Region
England
NLM ID
8208664
PMCID
PMC450106
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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