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PMID: 8606190 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An O-antigen processing function for Wzx (RfbX): a promising candidate for O-unit flippase.

Journal of bacteriology ·Vol. 178 ·No. 7 ·1996-04-00 ·Pages 2102-7

Liu D, Cole RA, Reeves PR

Abstract

O antigen is the major cell surface antigen of gram-negative bacteria, and the genes responsible for its synthesis are located in a single gene cluster. The wzx (rbfX) gene, which is characteristic of the major class of O-antigen gene clusters, encodes a hydrophobic protein with 12 potential transmembrane segments. We demonstrate that a wzx mutant accumulates undecaprenol pyrophosphate-linked O units which appear to be on the cytoplasmic side of the cytoplasmic membrane, suggesting that the wzx gene encodes a flippase for O-unit translocation across that membrane.

MeSH Terms
Bacterial Proteins/genetics,metabolism Carbohydrate Sequence Carrier Proteins/metabolism Gene Expression Regulation, Bacterial Membrane Proteins/genetics,metabolism Membrane Transport Proteins Molecular Sequence Data Multigene Family Mutation O Antigens/biosynthesis Phospholipid Transfer Proteins
Chemicals
Bacterial Proteins Carrier Proteins Membrane Proteins Membrane Transport Proteins O Antigens Phospholipid Transfer Proteins RfbX protein, Shigella flexneri
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Liu D
Department of Microbiology, University of Sydney, New South Wales, Australia.
Cole R A
Reeves P R
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28 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.7.2102-2107.1996
Published
1996-04-00
Pages
2102-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177911
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.178.7.2102-2107.1996 PMC Full Text View journal details
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