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PMID: 8605308 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Leaf senescence in Brassica napus: expression of genes encoding pathogenesis-related proteins.

Plant molecular biology ·Vol. 30 ·No. 3 ·1996-02-00 ·Pages 597-609

Hanfrey C, Fife M, Buchanan-Wollaston V

Abstract

Genes that are expressed during leaf senescence in Brassica napus were identified by the isolation of representative cDNA clones. DNA sequence and deduced protein sequence from two senescence-related cDNAs, LSC94 and LSC222, representing genes that are expressed early in leaf senescence before any yellowing of the leaves is visible, showed similarities to genes for pathogenesis-related (PR) proteins: a PR-1a-like protein and a class IV chitinase, respectively. The LSC94 and LSC222 genes showed differential regulation with respect to each other; an increase in expression was detected at different times during development of healthy leaves. Expression of both genes was induced by salicylic acid treatment. These findings suggest that some PR genes, as well as being induced by pathogen infection, may have alternative functions during plant development, for example in the process of leaf senescence.

MeSH Terms
Amino Acid Sequence Base Sequence Brassica/genetics,growth & development Chitinases/genetics,physiology DNA, Complementary DNA, Plant Gene Expression/drug effects Genes, Plant Molecular Sequence Data Plant Leaves/physiology Plant Proteins/chemistry,genetics,metabolism,physiology Salicylates/pharmacology Salicylic Acid Time Factors
Chemicals
DNA, Complementary DNA, Plant LSC222 protein, Brassica napus Plant Proteins Salicylates YPR1 protein, Brassica napus pathogenesis-related proteins, plant Chitinases Salicylic Acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hanfrey C
Plant Molecular Biology Laboratory, Department of Biological Sciences, Wye College, University of London, Ashford, Kent, UK.
Fife M
Buchanan-Wollaston V
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00049334
Published
1996-02-00
Pages
597-609
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
U21848, U21849
External Links
PubMed source DOI: 10.1007/BF00049334 View journal details
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