Isolation of cDNA clones for genes that are expressed during leaf senescence in Brassica napus. Identification of a gene encoding a senescence-specific metallothionein-like protein.

Plant physiology ·Vol. 105 ·No. 3 ·1994-07-00 ·Pages 839-46

Buchanan-Wollaston V

Abstract

cDNA clones representing genes that are expressed during leaf senescence in Brassica napus were identified by differential screening of a cDNA library made from RNA isolated from leaves at different stages of senescence. The expression of these genes at different stages of leaf development was examined by northern blot analysis, and several different patterns of expression were observed. One of the clones, LSC54, represented a gene that is expressed at high levels during leaf senescence. Analysis of this gene indicated strong expression in flowers as well as in senescing leaves. DNA sequence analysis of the LSC54 cDNA indicated a similarity between the deduced amino acid sequence and several metallothionein-like proteins previously identified in plants.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Brassica/genetics,growth & development Cloning, Molecular DNA Primers DNA, Complementary/isolation & purification Gene Expression Gene Library Genes, Plant Metallothionein/chemistry,genetics Molecular Sequence Data Polymerase Chain Reaction Protein Biosynthesis Rabbits Reticulocytes/metabolism Sequence Homology, Amino Acid

Chemicals

DNA Primers DNA, Complementary Metallothionein

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Buchanan-Wollaston V

Department of Biological Sciences, Wye College, University of London, Ashford, Kent, United Kingdom.

References (8)

8 references, click to expand

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Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

0032-0889

DOI

10.1104/pp.105.3.839

Published

1994-07-00

Pages

839-46

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC160730

Subset

Databases

GENBANK

U20236

External Links

PubMed source DOI: 10.1104/pp.105.3.839 PMC Full Text View journal details

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Isolation of cDNA clones for genes that are expressed during leaf senescence in Brassica napus. Identification of a gene encoding a senescence-specific metallothionein-like protein.

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