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PMID: 8604311 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Structural characterization of U*-1915 in domain IV from Escherichia coli 23S ribosomal RNA as 3-methylpseudouridine.

Nucleic acids research ·Vol. 24 ·No. 4 ·1996-02-15 ·Pages 688-93

Kowalak JA, Bruenger E, Hashizume T, Peltier JM, Ofengand J, McCloskey JA

Abstract

Mass spectrometry-based methods have been used to study post-transcriptional modification in the 1900-1974 nt segment of domain IV in 23S rRNA of Escherichia coli, a region which interacts with domain V in forming the three- dimensional structure of the peptidyl transferase center within the ribosome. A nucleoside constituent of M r 258 (U*)which occurs at position 1915, within the highly modified oligonucleotide sequence 1911-psiAACU*Apsi-1917, was characterized as 3-methylpseudouridine (m3psi). The assignment was confirmed by chemical synthesis of m3psi and comparison with the natural nucleoside by liquid chromatography-mass spectrometry. 3-Methylpseudouridine is previously unknown in nature and is the only known derivative of the common modified nucleoside pseudouridine thus far found in bacterial rRNA.

MeSH Terms
Base Sequence Escherichia coli/chemistry Mass Spectrometry Molecular Sequence Data Molecular Structure Pseudouridine/analogs & derivatives,chemistry RNA, Ribosomal, 23S/chemistry
Chemicals
RNA, Ribosomal, 23S Pseudouridine 3-methylpseudouridine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kowalak J A
Department of Biochemistry, University of Utah, Salt Lake City, UT 84132, USA.
Bruenger E
Hashizume T
Peltier J M
Ofengand J
McCloskey J A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/24.4.688
Published
1996-02-15
Pages
688-93
Language
English
Region
England
NLM ID
0411011
PMCID
PMC145692
Subset
IM
Grants
NCI NIH HHS · 5P30CA42014 · United States
NIGMS NIH HHS · GM29812 · United States
External Links
PubMed source DOI: 10.1093/nar/24.4.688 PMC Full Text View journal details
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