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PMID: 8598204 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Editing of the GLuR-B ion channel RNA in vitro by recombinant double-stranded RNA adenosine deaminase.

The EMBO journal ·Vol. 15 ·No. 1 ·1996-01-02 ·Pages 34-45

Dabiri GA, Lai F, Drakas RA, Nishikura K

Abstract

Double-stranded RNA (dsRNA)-specific adenosine deaminase (DRADA) has been implicated as an enzyme responsible for the editing of RNA transcripts encoding glutamate-gated ion channel subunits (GLuR) in brain. In one case, the editing alters the gene-encoded glutamine (Q) to an arginine (R) located within the channel-forming domain of the alpha-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA) receptor subunit GLuR-B. The result of editing at this site, called the 'Q/R' site, is a profound alteration of the Ca2+ permeability of the GLuR channel. Using recombinantly expressed DRADA proteins, we now demonstrate in vitro that DRADA is indeed involved in editing of the GLuR-B RNA. In addition to the formation of an RNA duplex structure involving exon and intron sequences, Q/R site-selective editing by DRADA also requires a cofactor protein(s) commonly present even in non-neuronal cells. The accuracy and efficiency of this RNA editing system appear to be determined by the quantitative balance between DRADA, cofactor and substrate GLuR-B RNA.

MeSH Terms
Adenosine Deaminase/metabolism Base Sequence Cell Line Humans Introns Molecular Sequence Data Nuclear Proteins/metabolism Nucleic Acid Conformation RNA Editing RNA-Binding Proteins Receptors, AMPA/genetics
Chemicals
Nuclear Proteins RNA-Binding Proteins Receptors, AMPA ADARB1 protein, human Adenosine Deaminase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dabiri G A
The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA.
Lai F
Drakas R A
Nishikura K
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-01-02
Pages
34-45
Language
English
Region
England
NLM ID
8208664
PMCID
PMC449915
Subset
IM
Grants
NCI NIH HHS · CA09171 · United States
NCI NIH HHS · CA10815 · United States
NIGMS NIH HHS · GM40536 · United States
External Links
PubMed source PMC Full Text View journal details
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