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PMID: 8580344 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Single cytoplasmic dynein molecule movements: characterization and comparison with kinesin.

Biophysical journal ·Vol. 69 ·No. 5 ·1995-11-00 ·Pages 2011-23

Wang Z, Khan S, Sheetz MP

Abstract

Cytoplasmic dynein is a major microtubule motor for minus-end directed movements including retrograde axonal transport. To better understand the mechanism by which cytoplasmic dynein converts ATP energy into motility, we have analyzed the nanometer-level displacements of latex beads coated with low numbers of cytoplasmic dynein molecules. Cytoplasmic dynein-coated beads exhibited greater lateral movements among microtubule protofilaments (ave. 5.1 times/microns of displacement) compared with kinesin (ave. 0.9 times/micron). In addition, dynein moved rearward up to 100 nm over several hundred milliseconds, often in correlation with off-axis movements from one protofilament to another. We suggest that single molecules of cytoplasmic dynein move the beads because 1) there is a linear dependence of bead motility on dynein/bead ratio, 2) the binding of beads to microtubules studied by laser tweezers is best fit by a first-order Poisson, and 3) the run length histogram of dynein beads follows a first-order decay. At the cellular level, the greater disorder of cytoplasmic dynein movements may facilitate transport by decreasing the duration of collisions between kinesin and cytoplasmic dynein-powered vesicles.

MeSH Terms
Adenosine Triphosphate/metabolism Animals Axonal Transport/physiology Biophysical Phenomena Biophysics Chick Embryo Cytoplasm/chemistry,physiology Dyneins/chemistry,physiology Energy Metabolism In Vitro Techniques Kinesins/chemistry,physiology Latex Microspheres Movement/physiology
Chemicals
Latex Adenosine Triphosphate Dyneins Kinesins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wang Z
Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Khan S
Sheetz M P
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37 references, click to expand
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(95)80071-8
Published
1995-11-00
Pages
2011-23
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1236434
Subset
IM
Grants
NIGMS NIH HHS · GM36936 · United States
NINDS NIH HHS · NS23345 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(95)80071-8 PMC Full Text View journal details
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