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PMID: 2974459 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.

The Journal of cell biology ·Vol. 107 ·No. 6 Pt 2 ·1988-12-00 ·Pages 2657-67

Ingold AL, Cohn SA, Scholey JM

Abstract

We have prepared and characterized seven mouse monoclonal antibodies (SUK 1-7) to the 130-kD heavy chain of sea urchin egg kinesin. On immunoblots, SUK 3 and SUK 4 cross-reacted with Drosophila embryo 116-kD heavy chains, and SUK 4, SUK 5, SUK 6, and SUK 7 bound to the 120-kD heavy chains of bovine brain kinesin. Three out of seven monoclonal antikinesins (SUK 4, SUK 6, and SUK 7) caused a dose-dependent inhibition of sea urchin egg kinesin-induced microtubule translocation, whereas the other four monoclonal antibodies had no detectable effect on this motility. The inhibitory monoclonal antibodies (SUK 4, SUK 6, and SUK 7) appear to bind to spatially related sites on an ATP-sensitive microtubule binding 45-kD chymotryptic fragment of the 130-kD heavy chain, whereas SUK 2 binds to a spatially distinct site. None of the monoclonal antikinesins inhibited the microtubule activated MgATPase activity of kinesin, suggesting that SUK 4, SUK 6, and SUK 7 uncouple this MgATPase activity from motility.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Binding, Competitive Ca(2+) Mg(2+)-ATPase/immunology,metabolism Chromatography, Gel Cross Reactions Electrophoresis, Polyacrylamide Gel Hybridomas Immunoblotting Kinesins Mice Microtubule Proteins/immunology,physiology Microtubules/physiology Nerve Tissue Proteins/immunology,physiology Radioimmunoassay Sea Urchins
Chemicals
Antibodies, Monoclonal Microtubule Proteins Nerve Tissue Proteins Ca(2+) Mg(2+)-ATPase Kinesins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ingold A L
Department of Molecular and Cellular Biology, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80302.
Cohn S A
Scholey J M
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34 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.107.6.2657
Published
1988-12-00
Pages
2657-67
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115674
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.107.6.2657 PMC Full Text View journal details
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