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PMID: 8573788 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Beta 1 integrins signal lipid second messengers required during cell adhesion.

Molecular biology of the cell ·Vol. 6 ·No. 10 ·1995-10-00 ·Pages 1305-13

Auer KL, Jacobson BS

Abstract

Clustering of integrin receptors during cell adhesion stimulates signal transduction across the cell membrane. Second messengers are generated, activating cytosolic proteins and causing cytoskeletal assembly and rearrangement. HeLa cell adhesion to a collagen substrate has been shown to initiate an arachidonic acid-mediated signaling pathway, leading to the activation of protein kinase C (PKC) and cell spreading. To determine the role of integrin receptors in triggering this signaling pathway, monoclonal antibodies to beta 1 integrins were used to either cluster integrins on the cell surface or to provide an integrin-dependent substrate for cell adhesion. Using this approach, we have defined a pathway required for cell spreading that can be initiated by the ligation of integrins and leads to the activation of PKC. Specifically, our results indicate that clustering beta 1 integrins results in the activation of phospholipase A2 leading to the production of arachidonic acid and the activation of PKC.

MeSH Terms
Acetophenones/pharmacology Antibodies, Monoclonal Arachidonic Acid/metabolism,physiology Cell Adhesion/physiology Cell Membrane/metabolism Enzyme Activation Enzyme Inhibitors/pharmacology Gelatin HeLa Cells Humans Integrin beta1/metabolism,physiology Lipoxygenase Inhibitors/pharmacology Oxidation-Reduction Phospholipases A/antagonists & inhibitors Phospholipases A2 Protein Kinase C/physiology Second Messenger Systems/physiology
Chemicals
Acetophenones Antibodies, Monoclonal Enzyme Inhibitors Integrin beta1 Lipoxygenase Inhibitors Arachidonic Acid Gelatin Protein Kinase C Phospholipases A Phospholipases A2 4-bromophenacyl bromide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Auer K L
Department of Biochemistry and Molecular Biology, University of Massachusetts at Amherst 01003, USA.
Jacobson B S
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.6.10.1305
Published
1995-10-00
Pages
1305-13
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC301289
Subset
IM
Grants
NHGRI NIH HHS · NIHGM-29127 · United States
External Links
PubMed source DOI: 10.1091/mbc.6.10.1305 PMC Full Text View journal details
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