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PMID: 8557369 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Localization of biologically important regions on toxic shock syndrome toxin 1.

Infection and immunity ·Vol. 64 ·No. 1 ·1996-01-00 ·Pages 371-4

Murray DL, Earhart CA, Mitchell DT, Ohlendorf DH, Novick RP, Schlievert PM

Abstract

Toxic shock syndrome toxin 1 (TSST-1) contains a long central alpha helix that forms the base of two grooves on opposite sides of the molecule. Previous studies indicated that residues 132, 135, and 140 along the back of the central alpha helix are important in the biological activities. We made mutations of additional central alpha-helix residues exposed along this groove on the back of TSST-1. The proteins were purified, shown not to have gross alteration in structure, and tested for both superantigenicity and ability to elicit lethal TSS, using the superantigenicity, likely to because of alteration in T-cell receptor binding. Mutants H135A, Q136A, and E132K/ Q136K lost the ability to induce lethal TSS. The mutant Q136A was most increasing because it was superantigenic, yet nonlethal.

MeSH Terms
Animals Bacterial Toxins DNA Mutational Analysis Dose-Response Relationship, Drug Enterotoxins/genetics,pharmacology Lymphocyte Activation/drug effects Models, Molecular Mutagenesis, Site-Directed Protein Structure, Secondary Rabbits Shock, Septic Staphylococcus aureus/immunology Structure-Activity Relationship Superantigens/genetics,pharmacology
Chemicals
Bacterial Toxins Enterotoxins Superantigens enterotoxin F, Staphylococcal
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Murray D L
Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455, USA.
Earhart C A
Mitchell D T
Ohlendorf D H
Novick R P
Schlievert P M
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.64.1.371-374.1996
Published
1996-01-00
Pages
371-4
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC173772
Subset
IM
Grants
NIAID NIH HHS · AI22159 · United States
External Links
PubMed source DOI: 10.1128/iai.64.1.371-374.1996 PMC Full Text View journal details
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