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PMID: 8552669 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning and characterization of a binding subunit of the interleukin 13 receptor that is also a component of the interleukin 4 receptor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 1 ·1996-01-09 ·Pages 497-501

Hilton DJ, Zhang JG, Metcalf D, Alexander WS, Nicola NA, Willson TA

Abstract

Interleukins 4 (IL-4) and 13 (IL-13) have been found previously to share receptor components on some cells, as revealed by receptor cross-competition studies. In the present study, the cloning is described of murine NR4, a previously unrecognized receptor identified on the basis of sequence similarity with members of the hemopoietin receptor family. mRNA encoding NR4 was found in a wide range of murine cells and tissues. By using transient expression in COS-7 cells, NR4 was found to encode the IL-13 receptor alpha chain, a low-affinity receptor capable of binding IL-13 but not IL-4 or interleukins 2, -7, -9, or -15. Stable expression of the IL-13 receptor alpha chain (NR4) in CTLL-2 cells resulted in the generation of high-affinity IL-13 receptors capable of transducing a proliferative signal in response to IL-13 and, moreover, led to competitive cross-reactivity in the binding of IL-4 and IL-13. These results suggest that the IL-13 receptor alpha chain (NR4) is the primary binding subunit of the IL-13 receptor and may also be a component of IL-4 receptors.

MeSH Terms
Amino Acid Sequence Animals Antigens, CD/chemistry,genetics Base Sequence Cell Division Cells, Cultured DNA, Complementary/genetics Genes Interleukin-13/metabolism Interleukin-13 Receptor alpha1 Subunit Interleukin-4/metabolism Mice Molecular Sequence Data Receptors, Interleukin/chemistry,genetics,metabolism Receptors, Interleukin-13 Receptors, Interleukin-4 Recombinant Proteins/metabolism Transfection
Chemicals
Antigens, CD DNA, Complementary Il13ra1 protein, mouse Interleukin-13 Interleukin-13 Receptor alpha1 Subunit Receptors, Interleukin Receptors, Interleukin-13 Receptors, Interleukin-4 Recombinant Proteins Interleukin-4
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hilton D J
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
Zhang J G
Metcalf D
Alexander W S
Nicola N A
Willson T A
References (29)
29 references, click to expand
  1. Characterization and comparison of the interleukin 13 receptor with the interleukin 4 receptor on several cell types.
    J Biol Chem. 1995 Feb 24;270(8):3512-7 PMID: 7876085
  2. Oncostatin M and leukemia inhibitory factor trigger overlapping and different signals through partially shared receptor complexes.
    J Biol Chem. 1994 Feb 25;269(8):6215-22 PMID: 8119965
  3. The role of shared receptor motifs and common Stat proteins in the generation of cytokine pleiotropy and redundancy by IL-2, IL-4, IL-7, IL-13, and IL-15.
    Immunity. 1995 Apr;2(4):331-9 PMID: 7719938
  4. Receptor for interleukin 13. Interaction with interleukin 4 by a mechanism that does not involve the common gamma chain shared by receptors for interleukins 2, 4, 7, 9, and 15.
    J Biol Chem. 1995 Apr 14;270(15):8797-804 PMID: 7721786
  5. The primary binding subunit of the human interleukin-4 receptor is also a component of the interleukin-13 receptor.
    J Biol Chem. 1995 Jun 9;270(23):13869-78 PMID: 7775445
  6. Iodine monochloride (IC1) iodination techniques.
    Methods Enzymol. 1983;92:277-92 PMID: 6855615
  7. Functional participation of the IL-2 receptor gamma chain in IL-7 receptor complexes.
    Science. 1994 Mar 11;263(5152):1453-4 PMID: 8128231
  8. Interleukin 13, an interleukin 4-like cytokine that acts on monocytes and B cells, but not on T cells.
    Immunol Today. 1994 Jan;15(1):19-26 PMID: 7907877
  9. Utilization of the beta and gamma chains of the IL-2 receptor by the novel cytokine IL-15.
    EMBO J. 1994 Jun 15;13(12):2822-30 PMID: 8026467
  10. Cloning of a murine IL-11 receptor alpha-chain; requirement for gp130 for high affinity binding and signal transduction.
    EMBO J. 1994 Oct 17;13(20):4765-75 PMID: 7957045
  11. Design of human interleukin-4 antagonists inhibiting interleukin-4-dependent and interleukin-13-dependent responses in T-cells and B-cells with high efficiency.
    Eur J Biochem. 1994 Oct 15;225(2):659-65 PMID: 7957181
  12. Function of the interleukin-2 (IL-2) receptor gamma-chain in biologic responses of X-linked severe combined immunodeficient B cells to IL-2, IL-4, IL-13, and IL-15.
    Blood. 1995 Jan 1;85(1):38-42 PMID: 7803808
  13. Both interleukin 4 and interleukin 13 induce tyrosine phosphorylation of the 140-kDa subunit of the interleukin 4 receptor.
    J Biol Chem. 1995 Jan 13;270(2):966-70 PMID: 7822337
  14. Expression and function of the gamma c subunit of the IL-2, IL-4, and IL-7 receptors. Distinct interaction of gamma c in the IL-4 receptor.
    J Immunol. 1995 Feb 15;154(4):1596-605 PMID: 7530740
  15. Cloning and sequencing of a deoxyribonucleic acid copy of glyceraldehyde-3-phosphate dehydrogenase messenger ribonucleic acid isolated from chicken muscle.
    Biochemistry. 1983 Mar 29;22(7):1605-13 PMID: 6303388
  16. A potent human interleukin-4 antagonist stimulates the proliferation of murine cells expressing the human interleukin-4 binding chain.
    Growth Factors. 1995;12(1):69-83 PMID: 8527165
  17. The murine interleukin-4 receptor: molecular cloning and characterization of secreted and membrane bound forms.
    Cell. 1989 Oct 20;59(2):335-48 PMID: 2805066
  18. pEF-BOS, a powerful mammalian expression vector.
    Nucleic Acids Res. 1990 Sep 11;18(17):5322 PMID: 1698283
  19. Oncostatin M binds the high-affinity leukemia inhibitory factor receptor.
    New Biol. 1992 Jan;4(1):61-5 PMID: 1536831
  20. The IL-6 signal transducer, gp130: an oncostatin M receptor and affinity converter for the LIF receptor.
    Science. 1992 Mar 13;255(5050):1434-7 PMID: 1542794
  21. Kinetic analyses of the binding of leukemia inhibitory factor to receptor on cells and membranes and in detergent solution.
    J Biol Chem. 1992 May 25;267(15):10238-47 PMID: 1587813
  22. Cloning of the gamma chain of the human IL-2 receptor.
    Science. 1992 Jul 17;257(5068):379-82 PMID: 1631559
  23. Receptors for interleukin-13 and interleukin-4 are complex and share a novel component that functions in signal transduction.
    EMBO J. 1993 Jul;12(7):2663-70 PMID: 8101483
  24. An interleukin 4 (IL-4) mutant protein inhibits both IL-4 or IL-13-induced human immunoglobulin G4 (IgG4) and IgE synthesis and B cell proliferation: support for a common component shared by IL-4 and IL-13 receptors.
    J Exp Med. 1993 Dec 1;178(6):2213-8 PMID: 7504061
  25. Sharing of the interleukin-2 (IL-2) receptor gamma chain between receptors for IL-2 and IL-4.
    Science. 1993 Dec 17;262(5141):1874-7 PMID: 8266076
  26. Interleukin-2 receptor gamma chain: a functional component of the interleukin-7 receptor.
    Science. 1993 Dec 17;262(5141):1877-80 PMID: 8266077
  27. Interleukin-2 receptor gamma chain: a functional component of the interleukin-4 receptor.
    Science. 1993 Dec 17;262(5141):1880-3 PMID: 8266078
  28. Histidine-367 of the human common beta chain of the receptor is critical for high-affinity binding of human granulocyte-macrophage colony-stimulating factor.
    Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):252-6 PMID: 8278375
  29. Sharing of the IL-2 receptor gamma chain with the functional IL-9 receptor complex.
    Int Immunol. 1995 Jan;7(1):115-20 PMID: 7718508
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.1.497
Published
1996-01-09
Pages
497-501
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC40265
Subset
IM
Grants
NCI NIH HHS · CA-22556 · United States
Databases
GENBANK
S80963
External Links
PubMed source DOI: 10.1073/pnas.93.1.497 PMC Full Text View journal details
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