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PMID: 7957045 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning of a murine IL-11 receptor alpha-chain; requirement for gp130 for high affinity binding and signal transduction.

The EMBO journal ·Vol. 13 ·No. 20 ·1994-10-17 ·Pages 4765-75

Hilton DJ, Hilton AA, Raicevic A, Rakar S, Harrison-Smith M, Gough NM, Begley CG, Metcalf D, Nicola NA, Willson TA

Abstract

An adult mouse liver cDNA library was screened with oligonucleotides corresponding to the conserved WSXWS motif of the haemopoietin receptor family. Using this method, cDNA clones encoding a novel receptor were isolated. The new receptor, named NR1, was most similar in sequence and predicted structure to the alpha-chain of the IL-6 receptor and mRNA was expressed in the 3T3-L1 pre-adipocytic cell line and in a range of primary tissues. Expression of NR1 in the factor-dependent haemopoietic cell line Ba/F3 resulted in the generation of low affinity receptors for IL-11 (Kd approximately 10 nM). The capacity to bind IL-11 with high affinity (Kd = 300-800 pM) appeared to require coexpression of both NR1 and gp130, the common subunit of the IL-6, leukaemia inhibitory factor (LIF), oncostatin M (OSM) and ciliary neurotrophic factor (CNTF) receptors. The expression of both NR1 and gp130 was also necessary for Ba/F3 cells to proliferate and M1 cells to undergo macrophage differentiation in response to IL-11.

MeSH Terms
3T3 Cells Amino Acid Sequence Animals Antigens, CD Base Sequence Cell Division/genetics Cell Line Cloning, Molecular Cytokine Receptor gp130 DNA, Complementary Interleukin-11 Receptor alpha Subunit Membrane Glycoproteins/genetics,metabolism,physiology Mice Molecular Sequence Data RNA, Messenger/genetics Receptors, Interleukin/chemistry,metabolism,physiology Receptors, Interleukin-11 Sequence Homology, Amino Acid Signal Transduction
Chemicals
Antigens, CD DNA, Complementary Il11ra1 protein, mouse Il11ra2 protein, mouse Il6st protein, mouse Interleukin-11 Receptor alpha Subunit Membrane Glycoproteins RNA, Messenger Receptors, Interleukin Receptors, Interleukin-11 Cytokine Receptor gp130
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Hilton D J
Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria.
Hilton A A
Raicevic A
Rakar S
Harrison-Smith M
Gough N M
Begley C G
Metcalf D
Nicola N A
Willson T A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-10-17
Pages
4765-75
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395415
Subset
IM
Grants
NCI NIH HHS · CA-22556 · United States
Databases
GENBANK
U14412
External Links
PubMed source PMC Full Text View journal details
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