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PMID: 8552099 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Schizosaccharomyces pombe pcr1+ encodes a CREB/ATF protein involved in regulation of gene expression for sexual development.

Molecular and cellular biology ·Vol. 16 ·No. 2 ·1996-02-00 ·Pages 704-11

Watanabe Y, Yamamoto M

Abstract

The Schizosaccharomyces pombe pcr1 gene encodes a bZIP protein that apparently belongs to the cyclic AMP response element (CRE)-binding protein/activating transcription factor family. The deduced pcr1 gene product consists of 171 amino acid residues and is most similar to the mammalian CRE-BP1. A glutathione S-transferase-Pcr1 fusion protein produced in Escherichia coli was able to bind specifically to the CRE motif in vitro. Analysis with anti-Pcr1 serum suggested that Pcr1 is included in the major CRE-binding factors present in the S. pombe cell extract. Disruption of the pcr1 gene was not lethal, but the disruptant showed cold-sensitive growth on rich medium. The disruptant was also inefficient in mating and sporulation, though it was not completely sterile. Expression of the ste11 gene, which encodes a key transcription factor for sexual development, was greatly reduced in the disruptant, and overexpression of ste11+ suppressed the deficiency of the pcr1 disruptant in sexual development. It has been shown that expression of ste11 is negatively regulated by cyclic AMP-dependent protein kinase (PKA) and that the loss of PKA activity results in ectopic sexual development. Disruption of pcr1 blocked ectopic sexual development. Furthermore, disruption of pcr1 reduced expression of fbp1, a glucose-repressible gene negatively regulated by PKA. These results suggest that Pcr1 is a putative transcriptional regulator whose activity may be controlled by PKA. Alternatively, its activity may be independent of PKA, and full induction of ste11 and fbp1 expression requires the function of Pcr1 in addition to elimination of the repression by PKA.

MeSH Terms
Activating Transcription Factors Amino Acid Sequence Base Sequence Basic-Leucine Zipper Transcription Factors Binding Sites Blood Proteins Blotting, Northern Cloning, Molecular Cyclic AMP/metabolism Cyclic AMP Response Element-Binding Protein DNA-Binding Proteins/genetics,metabolism G-Box Binding Factors Gene Expression Regulation, Fungal Genes, Fungal Leucine Zippers Molecular Sequence Data Mutagenesis Phenotype Protein Binding Reproduction Schizosaccharomyces/genetics,growth & development Schizosaccharomyces pombe Proteins Sequence Analysis, DNA Sequence Homology, Amino Acid Signal Transduction Transcription Factors/genetics,metabolism
Chemicals
Activating Transcription Factors Basic-Leucine Zipper Transcription Factors Blood Proteins Cyclic AMP Response Element-Binding Protein DNA-Binding Proteins G-Box Binding Factors Pcr1 protein, S pombe Schizosaccharomyces pombe Proteins Transcription Factors Cyclic AMP
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Watanabe Y
Department of Biophysics and Biochemistry, School of Science, University of Tokyo, Japan.
Yamamoto M
References (55)
55 references, click to expand
  1. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
    Gene. 1984 Jun;28(3):351-9 PMID: 6235151
  2. Schizosaccharomyces pombe pac2+ controls the onset of sexual development via a pathway independent of the cAMP cascade.
    Curr Genet. 1995 Jun;28(1):32-8 PMID: 8536311
  3. Rapid and efficient site-specific mutagenesis without phenotypic selection.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92 PMID: 3881765
  4. Rapid and sensitive protein similarity searches.
    Science. 1985 Mar 22;227(4693):1435-41 PMID: 2983426
  5. Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.
    Proc Natl Acad Sci U S A. 1986 Sep;83(18):6682-6 PMID: 2875459
  6. Nucleoprotein complexes that regulate gene expression in adipocyte differentiation: direct participation of c-fos.
    Cell. 1987 Jun 19;49(6):835-44 PMID: 3555845
  7. Analysis and in vivo disruption of the gene coding for calmodulin in Schizosaccharomyces pombe.
    Proc Natl Acad Sci U S A. 1987 Jun;84(11):3580-4 PMID: 3035538
  8. The DNA-binding domains of the jun oncoprotein and the yeast GCN4 transcriptional activator protein are functionally homologous.
    Cell. 1987 Sep 11;50(6):841-6 PMID: 3040261
  9. Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1.
    Science. 1987 Dec 4;238(4832):1386-92 PMID: 2825349
  10. Characterization of the gene for fructose-1,6-bisphosphatase from Saccharomyces cerevisiae and Schizosaccharomyces pombe. Sequence, protein homology, and expression during growth on glucose.
    J Biol Chem. 1988 May 5;263(13):6051-7 PMID: 2834361
  11. Interaction of a common cellular transcription factor, ATF, with regulatory elements in both E1a- and cyclic AMP-inducible promoters.
    Proc Natl Acad Sci U S A. 1988 May;85(10):3396-400 PMID: 2835770
  12. Expression of the SV40 promoter in fission yeast: identification and characterization of an AP-1-like factor.
    Cell. 1988 May 20;53(4):659-67 PMID: 2836064
  13. The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins.
    Science. 1988 Jun 24;240(4860):1759-64 PMID: 3289117
  14. [In memoriam Dr. E.H. La Chapelle].
    Ned Tijdschr Geneeskd. 1966 Sep 10;110(37):1620-1 PMID: 5341763
  15. Premeiotic DNA synthesis in fission yeast.
    Exp Cell Res. 1974 Sep;88(1):127-34 PMID: 4472733
  16. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  17. Hac1: a novel yeast bZIP protein binding to the CRE motif is a multicopy suppressor for cdc10 mutant of Schizosaccharomyces pombe.
    Nucleic Acids Res. 1994 Dec 11;22(24):5279-88 PMID: 7816617
  18. Evidence for translational regulation of the activator of general amino acid control in yeast.
    Proc Natl Acad Sci U S A. 1984 Oct;81(20):6442-6 PMID: 6387704
  19. Analysis of FBJ-MuSV provirus and c-fos (mouse) gene reveals that viral and cellular fos gene products have different carboxy termini.
    Cell. 1983 Apr;32(4):1241-55 PMID: 6301687
  20. One-step gene disruption in yeast.
    Methods Enzymol. 1983;101:202-11 PMID: 6310324
  21. The S.pombe mei2 gene encoding a crucial molecule for commitment to meiosis is under the regulation of cAMP.
    EMBO J. 1988 Mar;7(3):761-7 PMID: 2840284
  22. Phosphorylation-induced binding and transcriptional efficacy of nuclear factor CREB.
    Nature. 1988 Aug 11;334(6182):494-8 PMID: 2900470
  23. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
    Gene. 1988 Jul 15;67(1):31-40 PMID: 3047011
  24. Fos and Jun: the AP-1 connection.
    Cell. 1988 Nov 4;55(3):395-7 PMID: 3141060
  25. Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA.
    Science. 1988 Dec 9;242(4884):1430-3 PMID: 2974179
  26. A family of immunologically related transcription factors that includes multiple forms of ATF and AP-1.
    Genes Dev. 1988 Oct;2(10):1216-26 PMID: 3144478
  27. Identification and purification of a Saccharomyces cerevisiae protein with the DNA binding specificity of mammalian activating transcription factor.
    Proc Natl Acad Sci U S A. 1989 Jan;86(1):109-13 PMID: 2643094
  28. Mammalian cAMP-responsive element can activate transcription in yeast and binds a yeast factor(s) that resembles the mammalian transcription factor ANF.
    Proc Natl Acad Sci U S A. 1989 Apr;86(7):2176-80 PMID: 2538834
  29. Genetic engineering of Schizosaccharomyces pombe: a system for gene disruption and replacement using the ura4 gene as a selectable marker.
    Mol Gen Genet. 1988 Dec;215(1):81-6 PMID: 3241624
  30. Yeast YAP1 encodes a novel form of the jun family of transcriptional activator proteins.
    Genes Dev. 1989 Mar;3(3):283-92 PMID: 2542125
  31. Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain.
    EMBO J. 1989 Jul;8(7):2023-8 PMID: 2529117
  32. Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.
    Genes Dev. 1989 Dec;3(12B):2083-90 PMID: 2516827
  33. mXBP/CRE-BP2 and c-Jun form a complex which binds to the cyclic AMP, but not to the 12-O-tetradecanoylphorbol-13-acetate, response element.
    Mol Cell Biol. 1990 Apr;10(4):1609-21 PMID: 2138707
  34. Transcription factors: a new family gathers at the cAMP response site.
    Trends Genet. 1990 Mar;6(3):69-72 PMID: 2158162
  35. Mutations that define the optimal half-site for binding yeast GCN4 activator protein and identify an ATF/CREB-like repressor that recognizes similar DNA sites.
    Mol Cell Biol. 1990 Oct;10(10):5077-86 PMID: 2204805
  36. Adenylyl cyclase is dispensable for vegetative cell growth in the fission yeast Schizosaccharomyces pombe.
    Proc Natl Acad Sci U S A. 1990 Oct;87(20):7814-8 PMID: 2172964
  37. High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe.
    Nucleic Acids Res. 1990 Nov 25;18(22):6485-9 PMID: 2251111
  38. Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases.
    Genes Dev. 1991 Jan;5(1):60-73 PMID: 1899230
  39. Activation of protein kinase C decreases phosphorylation of c-Jun at sites that negatively regulate its DNA-binding activity.
    Cell. 1991 Feb 8;64(3):573-84 PMID: 1846781
  40. Glucose repression of transcription of the Schizosaccharomyces pombe fbp1 gene occurs by a cAMP signaling pathway.
    Genes Dev. 1991 Apr;5(4):561-71 PMID: 1849107
  41. Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase.
    Anal Biochem. 1991 Feb 1;192(2):262-7 PMID: 1852137
  42. Highly conserved residues in the bZIP domain of yeast GCN4 are not essential for DNA binding.
    Mol Cell Biol. 1991 Oct;11(10):4918-26 PMID: 1922025
  43. Interaction between ran1+ protein kinase and cAMP dependent protein kinase as negative regulators of fission yeast meiosis.
    EMBO J. 1991 Dec;10(12):3759-68 PMID: 1657594
  44. Schizosaccharomyces pombe ste11+ encodes a transcription factor with an HMG motif that is a critical regulator of sexual development.
    Genes Dev. 1991 Nov;5(11):1990-9 PMID: 1657709
  45. Oncogenic and transcriptional cooperation with Ha-Ras requires phosphorylation of c-Jun on serines 63 and 73.
    Nature. 1991 Dec 12;354(6353):494-6 PMID: 1749429
  46. The wis1 protein kinase is a dosage-dependent regulator of mitosis in Schizosaccharomyces pombe.
    EMBO J. 1991 Dec;10(13):4291-9 PMID: 1756736
  47. In vivo footprinting of the human alpha-globin locus upstream regulatory element by guanine and adenine ligation-mediated polymerase chain reaction.
    Mol Cell Biol. 1992 May;12(5):2135-42 PMID: 1569944
  48. Reduction in the intracellular cAMP level triggers initiation of sexual development in fission yeast.
    Mol Gen Genet. 1992 May;233(1-2):17-24 PMID: 1318497
  49. Yeast SKO1 gene encodes a bZIP protein that binds to the CRE motif and acts as a repressor of transcription.
    Nucleic Acids Res. 1992 Oct 25;20(20):5271-8 PMID: 1437546
  50. ACR1, a yeast ATF/CREB repressor.
    Mol Cell Biol. 1992 Dec;12(12):5394-405 PMID: 1448073
  51. Thiamine-repressible expression vectors pREP and pRIP for fission yeast.
    Gene. 1993 Jan 15;123(1):127-30 PMID: 8422996
  52. Identification of an oncoprotein- and UV-responsive protein kinase that binds and potentiates the c-Jun activation domain.
    Genes Dev. 1993 Nov;7(11):2135-48 PMID: 8224842
  53. Cloning of the pka1 gene encoding the catalytic subunit of the cAMP-dependent protein kinase in Schizosaccharomyces pombe.
    J Biol Chem. 1994 Apr 1;269(13):9632-7 PMID: 8144551
  54. Structural modification of spindle pole bodies during meiosis II is essential for the normal formation of ascospores in Schizosaccharomyces pombe: ultrastructural analysis of spo mutants.
    Yeast. 1994 Feb;10(2):173-83 PMID: 8203159
  55. S. pombe mei2+ encodes an RNA-binding protein essential for premeiotic DNA synthesis and meiosis I, which cooperates with a novel RNA species meiRNA.
    Cell. 1994 Aug 12;78(3):487-98 PMID: 7520368
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.2.704
Published
1996-02-00
Pages
704-11
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231050
Subset
IM
Databases
GENBANK
D63667
External Links
PubMed source DOI: 10.1128/MCB.16.2.704 PMC Full Text View journal details
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