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PMID: 8551592 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Processing of rabbit hemorrhagic disease virus polyprotein.

Journal of virology ·Vol. 70 ·No. 2 ·1996-02-00 ·Pages 1261-5

Martín Alonso JM, Casais R, Boga JA, Parra F

Abstract

Expression of rabbit hemorrhagic disease virus (RHDV) cDNAs in vitro with rabbit reticulocyte lysates and in Escherichia coli have been used to study the proteolytic processing of RHDV polyprotein encoded by ORF1. An epitope tag was used for monitoring the gene products by a specific antibody. We have identified four gene products with molecular masses of 80, 43, 73, and 60 kDa, from the amino to the carboxy terminus of the polyprotein. The amino-terminal sequences of the 43- and 73-kDa products were determined and indicated that RHDV 3C proteinase cleaved Glu-Gly peptide bonds.

MeSH Terms
Animals Cysteine Endopeptidases/metabolism Hemorrhagic Disease Virus, Rabbit/metabolism Protein Processing, Post-Translational Proteins/metabolism Rabbits Viral Nonstructural Proteins/metabolism Viral Proteins/metabolism Viral Structural Proteins/metabolism
Chemicals
Proteins Viral Nonstructural Proteins Viral Proteins Viral Structural Proteins viral protein 60, rabbit hemorrhagic disease virus 3C-like protease, rabbit hemorrhagic disease virus Cysteine Endopeptidases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Martín Alonso J M
Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, Spain.
Casais R
Boga J A
Parra F
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.2.1261-1265.1996
Published
1996-02-00
Pages
1261-5
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC189940
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.70.2.1261-1265.1996 PMC Full Text View journal details
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