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PMID: 1731695 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Identification and sequence determination of the capsid protein gene of feline calicivirus.

Archives of virology ·Vol. 122 ·No. 3-4 ·1992-00-00 ·Pages 223-35

Carter MJ, Milton ID, Turner PC, Meanger J, Bennett M, Gaskell RM

Abstract

We have determined 4380 bases of the sequence from a cDNA clone containing the 3' end of feline calicivirus strain F9. We find four candidate open reading frames of which three are complete and comprise 245, 317 and 2012 nucleotides. The fourth continues toward the 5' end. We have expressed the largest complete open reading frame in E. coli. Sera raised to this antigen react specifically with the capsid protein and its intracellular precursor molecule. N-terminal sequence analysis of purified, mature capsid protein confirms this assignment and has identified the position at which precursor is cleaved.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Caliciviridae/genetics Capsid/genetics Cloning, Molecular DNA, Viral Genes, Viral Mice Molecular Sequence Data Open Reading Frames Protein Precursors/genetics Sequence Alignment
Chemicals
DNA, Viral Protein Precursors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Carter M J
Division of Virology, School of Pathological Sciences, New Medical School, Newcastle upon Tyne, U.K.
Milton I D
Turner P C
Meanger J
Bennett M
Gaskell R M
References (21)
21 references, click to expand
  1. Method to determine the reading frame of a protein from the purine/pyrimidine genome sequence and its possible evolutionary justification.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1596-600 PMID: 6940175
  2. Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins.
    EMBO J. 1984 Jun;3(6):1429-34 PMID: 6086324
  3. Serologic classification of feline caliciviruses by plaque-reduction neutralization and immunodiffusion.
    Am J Vet Res. 1975 Apr;36(4 Pt.1):353-6 PMID: 804838
  4. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.
    EMBO J. 1982;1(8):945-51 PMID: 6329717
  5. Feline calicivirus protein synthesis investigated by western blotting.
    Arch Virol. 1989;108(1-2):69-79 PMID: 2596975
  6. Further characterization of the virus-specific RNAs in feline calicivirus infected cells.
    Virus Res. 1988 Aug;11(1):59-72 PMID: 3176687
  7. Animal picornaviruses with a single major species of capsid protein.
    Biochem Biophys Res Commun. 1973 Nov 1;55(1):141-9 PMID: 4798778
  8. All subgenomic mRNAs of equine arteritis virus contain a common leader sequence.
    Nucleic Acids Res. 1990 Jun 11;18(11):3241-7 PMID: 2162519
  9. Identification of the human papilloma virus-1a E4 gene products.
    EMBO J. 1986 Feb;5(2):355-62 PMID: 3011404
  10. Neutralisation patterns among recent British and North American feline calicivirus isolates from different clinical origins.
    Vet Rec. 1990 Aug 11;127(6):125-7 PMID: 2171183
  11. Nucleotide sequence of a region of the feline calicivirus genome which encodes picornavirus-like RNA-dependent RNA polymerase, cysteine protease and 2C polypeptides.
    Virus Res. 1990 Nov;17(3):145-60 PMID: 2077782
  12. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
    Gene. 1984 Jun;28(3):351-9 PMID: 6235151
  13. A model for vesicular exanthema virus, the prototype of the calicivirus group.
    J Gen Virol. 1978 Jul;40(1):161-74 PMID: 567672
  14. Solubilization and immune-detection of beta-galactosidase hybrid proteins carrying foreign antigenic determinants.
    Nucleic Acids Res. 1983 Jun 25;11(12):4077-92 PMID: 6191278
  15. Neutralizing epitopes of feline calicivirus.
    Arch Virol. 1991;117(3-4):173-81 PMID: 1708228
  16. Caliciviridae.
    Intervirology. 1980;14(1):1-6 PMID: 7203966
  17. Monoclonal antibodies to feline calicivirus.
    J Gen Virol. 1989 Aug;70 ( Pt 8):2197-200 PMID: 2475582
  18. Coronavirus mRNA synthesis involves fusion of non-contiguous sequences.
    EMBO J. 1983;2(10):1839-44 PMID: 6196191
  19. Transcription of feline calicivirus RNA.
    Arch Virol. 1990;114(3-4):143-52 PMID: 2241572
  20. A reliable method for the recovery of DNA fragments from agarose and acrylamide gels.
    Anal Biochem. 1981 Apr;112(2):295-8 PMID: 6266279
  21. Serological relationships among feline caliciviruses.
    Infect Immun. 1974 Dec;10(6):1307-14 PMID: 4435957
Article Info
Journal
Archives of virology
Abbr.
Arch Virol
ISSN
0304-8608
DOI
10.1007/BF01317185
Published
1992-00-00
Pages
223-35
Language
English
Region
Austria
NLM ID
7506870
PMCID
PMC7086951
Subset
IM
Grants
Wellcome Trust · United Kingdom
Databases
GENBANK
L05509, L05510, L05930, S45636, S45637, S45639, S45640, S74244, S74250, S77457
External Links
PubMed source DOI: 10.1007/BF01317185 PMC Full Text View journal details
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