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PMID: 8550449 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

D-histidine utilization in Salmonella typhimurium is controlled by the leucine-responsive regulatory protein (Lrp).

Journal of bacteriology ·Vol. 178 ·No. 2 ·1996-01-00 ·Pages 327-31

Hecht K, Zhang S, Klopotowski T, Ames GF

Abstract

A new class of D-histidine-utilizing mutants which carry mutations in the gene encoding the leucine-responsive regulatory protein (Lrp) has been identified in Salmonella typhimurium. The lrp mutations arise as suppressors of mutations in the genes encoding the histidine permease which drastically decrease the level of histidine transport activity. However, the suppressor effect is not exerted by elevating the level of the permease. Rather, the properties of the suppressor mutants are consistent with the notion that the parent permease mutants transport D-histidine at a low level and that in the suppressor mutants D-histidine is utilized effectively through elevated levels of racemization. The enzymatic activity of D-alanine dehydrogenase (Dad) is shown to be elevated in the suppressor mutants and is a possible pathway of D-histidine utilization. The suppressor mutations are located in the helix-turn-helix region of Lrp.

MeSH Terms
ATP-Binding Cassette Transporters Alanine Dehydrogenase Amino Acid Oxidoreductases/metabolism Amino Acid Transport Systems, Basic Bacterial Proteins/genetics,physiology Biological Transport DNA-Binding Proteins/genetics,physiology Genes, Bacterial/genetics Genes, Suppressor/physiology Helix-Turn-Helix Motifs Histidine/metabolism Leucine-Responsive Regulatory Protein Membrane Transport Proteins/genetics Mutation Salmonella typhimurium/genetics,metabolism Stereoisomerism Transcription Factors
Chemicals
ATP-Binding Cassette Transporters Amino Acid Transport Systems, Basic Bacterial Proteins DNA-Binding Proteins Membrane Transport Proteins Transcription Factors Leucine-Responsive Regulatory Protein Histidine histidine permease, Bacteria Amino Acid Oxidoreductases Alanine Dehydrogenase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hecht K
Department of Molecular and Cell Biology, University of California at Berkeley 94720, USA.
Zhang S
Klopotowski T
Ames G F
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24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.2.327-331.1996
Published
1996-01-00
Pages
327-31
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177661
Subset
IM
Grants
NIDDK NIH HHS · DK12121 · United States
External Links
PubMed source DOI: 10.1128/jb.178.2.327-331.1996 PMC Full Text View journal details
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