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PMID: 8506322 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Synthetic peptides based on motifs present in human band 3 protein inhibit cytoadherence/sequestration of the malaria parasite Plasmodium falciparum.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 10 ·1993-05-15 ·Pages 4703-7

Crandall I, Collins WE, Gysin J, Sherman IW

Abstract

Synthetic peptides patterned on the amino acid sequences found in two exofacial regions of band 3 protein (residues 824-829 of loop 7 and residues 547-553 of loop 3) blocked, in a dose-dependent fashion, the in vitro adherence of Plasmodium falciparum-infected erythrocytes to C32 amelanotic melanoma cells. Intravenous infusion of these synthetic peptides into Aotus and Saimiri monkeys infected with sequestering isolates of P. falciparum resulted in the appearance of mature forms of the parasite in the peripheral circulation. The finding that the peptides were effective as adhesion blockers in the micromolar range suggests that cerebral malaria could be managed through antiadhesion therapy.

MeSH Terms
Amino Acid Sequence Animals Anion Exchange Protein 1, Erythrocyte/chemistry,metabolism Binding, Competitive Cell Adhesion/drug effects Erythrocytes/parasitology Molecular Sequence Data Peptides/chemistry,metabolism Plasmodium falciparum/growth & development Protein Structure, Secondary Structure-Activity Relationship
Chemicals
Anion Exchange Protein 1, Erythrocyte Peptides
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Crandall I
Department of Biology, University of California, Riverside 92521.
Collins W E
Gysin J
Sherman I W
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.10.4703
Published
1993-05-15
Pages
4703-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46581
Subset
IM
Grants
NIAID NIH HHS · AI-21251 · United States
External Links
PubMed source DOI: 10.1073/pnas.90.10.4703 PMC Full Text View journal details
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