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PMID: 1696010 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Definition of a physiologic aging autoantigen by using synthetic peptides of membrane protein band 3: localization of the active antigenic sites.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 15 ·1990-08-00 ·Pages 5734-8

Kay MM, Marchalonis JJ, Hughes J, Watanabe K, Schluter SF

Abstract

Senescent cell antigen (SCA), an aging antigen, is a protein that appears on old cells and marks them for removal by the immune system in mammals. It is derived from band 3, a ubiquitous membrane transport protein found in diverse cell types and tissues. We have used synthetic peptides to identify aging antigenic sites on band 3, using a competitive inhibition assay and immunoblotting with IgG directed against the aging antigen on old cells. Results indicate that: (i) the active antigenic sites of the aging antigen reside on membrane protein band 3 residues that are extracellular regions implicated in anion transport (residues 538-554 and 788-827); (ii) a putative ankyrin-binding-region peptide is not involved in SCA activity; and (iii) carbohydrate moieties are not required for the antigenicity or recognition of SCA because synthetic peptides alone abolish binding of senescent cell IgG to erythrocytes. One of the putative transport sites that contributes to the aging antigen is located toward the carboxyl terminus. A model of band 3 is presented. Localization of the active antigenic site on the band 3 molecule facilitates definition of the molecular changes occurring during aging that initiate molecular as well as cellular degeneration.

MeSH Terms
Amino Acid Sequence Anion Exchange Protein 1, Erythrocyte/immunology Antigen-Antibody Complex Antigens, Differentiation/analysis Binding, Competitive Electrophoresis, Polyacrylamide Gel Epitopes/analysis Erythrocyte Aging/immunology Erythrocyte Membrane/ultrastructure Humans Immunoglobulin G/isolation & purification Models, Molecular Molecular Sequence Data Peptides/chemical synthesis Protein Conformation
Chemicals
Anion Exchange Protein 1, Erythrocyte Antigen-Antibody Complex Antigens, Differentiation Epitopes Immunoglobulin G Peptides senescent cell differentiation antigen
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kay M M
Department of Medicine, Texas A&M University, Temple 76503.
Marchalonis J J
Hughes J
Watanabe K
Schluter S F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.15.5734
Published
1990-08-00
Pages
5734-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54402
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.87.15.5734 PMC Full Text View journal details
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