Home LiteratureArticle Details
PMID: 6196779 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Polypeptides immunologically related to band 3 are present in nucleated somatic cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 22 ·1983-11-00 ·Pages 6882-6

Kay MM, Tracey CM, Goodman JR, Cone JC, Bassel PS

Abstract

Band 3, the major transmembrane polypeptide of erythrocytes, mediates the exchange of anions (chloride and bicarbonate) across the membrane. We suspected that band 3 was present on nucleated somatic cells as well as erythrocytes because the senescent cell antigen that is immunologically related to band 3 is present on lymphocytes, platelets, adult liver cells, and embryonic kidney cells; and antibodies prepared against the senescent cell antigen isolated from leukocytes react with erythrocyte band 3. For this reason, we examined human fibroblasts, lung cells, neutrophils, mononuclear leukocytes, squamous epithelial (mouth) cells, lung squamous epithelial carcinoma, mouse neuroblastoma cells, and rat hepatocytes for immunoreactive forms of band 3 by using monospecific antibodies to erythrocyte band 3. The results demonstrated that polypeptides sharing common antigenic determinants with erythrocyte band 3 are present in nucleated somatic cells as determined by immunofluorescence, immunoelectron microscopy, and immunoautoradiography. Peptide mapping revealed substantial sequence homology between erythrocyte band 3 and the band 3-like protein of leukocytes. Immunofluorescence studies indicate that the band 3-like proteins in nucleated cells participate in antibody-induced cell surface capping.

MeSH Terms
Anion Exchange Protein 1, Erythrocyte/immunology Cell Line Cells, Cultured Epitopes/analysis Fibroblasts/analysis Fluorescent Antibody Technique Humans Male Membrane Proteins/analysis Microscopy, Electron Neoplasms/analysis Peptide Fragments/analysis Peptides/analysis,immunology Skin/analysis
Chemicals
Anion Exchange Protein 1, Erythrocyte Epitopes Membrane Proteins Peptide Fragments Peptides
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kay M M
Tracey C M
Goodman J R
Cone J C
Bassel P S
References (33)
33 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. D-glucosyl isothiocyanate, an affinity label for the glucose transport proteins of the human erythrocyte membrane.
    Biochem Biophys Res Commun. 1973 Sep 18;54(2):593-9 PMID: 4756788
  3. Membrane proteins related to anion permeability of human red blood cells. I. Localization of disulfonic stilbene binding sites in proteins involved in permeation.
    J Membr Biol. 1974;15(3):207-26 PMID: 4838037
  4. Multiple labelling technique used for kinetic studies of activated human B lymphocytes.
    Nature. 1975 Apr 3;254(5499):424-6 PMID: 163985
  5. Binding of cytochalasin B to a red cell membrane protein.
    Biochem Biophys Res Commun. 1974 Dec 23;61(4):1471-6 PMID: 4455264
  6. Proteolytic dissection of band 3, the predominant transmembrane polypeptide of the human erythrocyte membrane.
    Biochemistry. 1976 Mar 9;15(5):1153-61 PMID: 1252433
  7. A study of the relationship between inhibition of anion exchange and binding to the red blood cell membrane of 4,4'-diisothiocyano stilbene-2,2'-disulfonic acid (DIDS) and its dihydro derivative (H2DIDS).
    J Membr Biol. 1976 Oct 20;29(1-2):147-77 PMID: 978716
  8. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
    J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
  9. Colloidal gold, a useful marker for transmission and scanning electron microscopy.
    J Histochem Cytochem. 1977 Apr;25(4):295-305 PMID: 323352
  10. Interaction of the aldolase and the membrane of human erythrocytes.
    Biochemistry. 1977 Jun 28;16(13):2966-71 PMID: 18166
  11. Changes in allosteric properties of phosphofructokinase bound to erythrocyte membranes.
    J Biol Chem. 1977 Nov 10;252(21):7418-20 PMID: 144126
  12. Preparation and analysis of seven major, topographically defined fragments of band 3, the predominant transmembrane polypeptide of human erythrocyte membranes.
    Biochemistry. 1978 Apr 4;17(7):1216-22 PMID: 656385
  13. Functional properties of human hemoglobin bound to the erythrocyte membrane.
    Biochemistry. 1979 Mar 6;18(5):893-9 PMID: 420820
  14. Comparison of structure and function of human erythrocyte and human muscle actin.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):935-8 PMID: 284418
  15. Autoregulation of an antibody response via network-induced auto-anti-idiotype.
    J Exp Med. 1979 Jul 1;150(1):67-85 PMID: 312909
  16. The membrane attachment protein for spectrin is associated with band 3 in human erythrocyte membranes.
    Nature. 1979 Aug 9;280(5722):468-73 PMID: 379653
  17. Immunoreactive forms of human erythrocyte ankyrin are present in diverse cells and tissues.
    Nature. 1979 Oct 18;281(5732):597-9 PMID: 492324
  18. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  19. Age-related changes in the immune system of mice of eight medium and long-lived strains and hybrids. I. Organ, cellular, and activity changes.
    Mech Ageing Dev. 1979 Dec;11(5-6):295-346 PMID: 316845
  20. Characterization of the anion transport system in hepatocyte plasma membranes.
    J Biol Chem. 1980 Apr 10;255(7):2637-40 PMID: 7358691
  21. Association of glyceraldehyde-3-phosphate dehydrogenase with the human red cell membrane. A kinetic analysis.
    J Biol Chem. 1980 Jul 10;255(13):6314-21 PMID: 7391020
  22. Isolation of the phagocytosis-inducing IgG-binding antigen on senescent somatic cells.
    Nature. 1981 Feb 5;289(5797):491-4 PMID: 7464917
  23. Homeostatic removal of senescent murine erythrocytes by splenic macrophages.
    Exp Hematol. 1981 Mar;9(3):297-307 PMID: 7227479
  24. Cell surface labelling with gold colloid particulates: the use of avidin and staphylococcal protein A-coated gold in conjunction with biotin and fc-bearing ligands.
    J Microsc. 1981 Aug;123(Pt 2):215-26 PMID: 6173487
  25. Identification of a spectrin-like protein in nonerythroid cells.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12):7570-4 PMID: 6950399
  26. F-actin-binding and cross-linking properties of porcine brain fodrin, a spectrin-related molecule.
    J Biol Chem. 1982 Aug 25;257(16):9781-7 PMID: 7107591
  27. Brain spectrin, a membrane-associated protein related in structure and function to erythrocyte spectrin.
    Nature. 1982 Sep 9;299(5879):126-31 PMID: 7110333
  28. Erythroid spectrin, brain fodrin, and intestinal brush border proteins (TW-260/240) are related molecules containing a common calmodulin-binding subunit bound to a variant cell type-specific subunit.
    Proc Natl Acad Sci U S A. 1982 Jul;79(13):4002-5 PMID: 6955786
  29. A protein immunologically related to erythrocyte band 4.1 is found on stress fibres on non-erythroid cells.
    Nature. 1982 Oct 14;299(5884):648-50 PMID: 6750415
  30. Antigenicity, storage, and aging: physiologic autoantibodies to cell membrane and serum proteins and the senescent cell antigen.
    Mol Cell Biochem. 1982 Nov 26;49(2):65-85 PMID: 6185837
  31. Expression of the beta subunit of spectrin in nonerythroid cells.
    Proc Natl Acad Sci U S A. 1983 Jan;80(2):363-7 PMID: 6340099
  32. Involvement of spectrin in cell-surface receptor capping in lymphocytes.
    Proc Natl Acad Sci U S A. 1983 Mar;80(6):1626-30 PMID: 6572928
  33. Senescent cell antigen is immunologically related to band 3.
    Proc Natl Acad Sci U S A. 1983 Mar;80(6):1631-5 PMID: 6572929
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.22.6882
Published
1983-11-00
Pages
6882-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390090
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.22.6882 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2