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PMID: 6572928 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Involvement of spectrin in cell-surface receptor capping in lymphocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 6 ·1983-03-00 ·Pages 1626-30

Nelson WJ, Colaço CA, Lazarides E

Abstract

Human and mouse lymphocytes of T- and B-cell lineages express a protein (Mr, 240,000) that crossreacts with antibodies raised against chicken erythrocyte alpha-spectrin as judged by immunofluorescence, immunoprecipitation, and immunoautoradiography; by the same criteria, antibodies raised against chicken erythrocyte beta-spectrin do not react with any lymphocyte polypeptide. In all T and B cells analyzed, before surface-directed ligand challenge with concanavalin A and surface immunoglobulins the polypeptide antigenically related to erythrocyte alpha-spectrin is distributed diffusely at the plasma membrane. Upon challenge, the redistribution of this polypeptide is concurrent with that of the cell-surface receptors initially in patches and then in a cap. Immunoprecipitation of NaDodSO4-solubilized lymphocytes with erythrocyte alpha-spectrin antiserum shows that in all cases a polypeptide with the same apparent molecular weight as erythrocyte alpha-spectrin is precipitated. Variable amounts of another polypeptide (Mr, 235,000) are also coimmunoprecipitated. Immunoprecipitations and subsequent immunoautoradiography show that the lymphocyte polypeptide doublet has a composition similar to that of (brain) fodrin, a polypeptide doublet that previously has been found mainly in the cells of nervous tissue.

MeSH Terms
Cell Membrane/physiology Cytoskeleton/physiology Immunologic Capping Lymphocytes/physiology Macromolecular Substances Membrane Proteins/physiology Molecular Weight Spectrin/immunology,physiology
Chemicals
Macromolecular Substances Membrane Proteins Spectrin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nelson W J
Colaço C A
Lazarides E
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.6.1626
Published
1983-03-00
Pages
1626-30
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393655
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.6.1626 PMC Full Text View journal details
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