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PMID: 8506279 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The LIM motif defines a specific zinc-binding protein domain.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 10 ·1993-05-15 ·Pages 4404-8

Michelsen JW, Schmeichel KL, Beckerle MC, Winge DR

Abstract

The cysteine-rich protein (CRP) contains two copies of the LIM sequence motif, CX2CX17HX2CX2CX2CX17-CX2C, that was first identified in the homeodomain proteins Lin-11, Is1-1, and Mec-3. The abundance and spacing of the cysteine residues in the LIM motif are reminiscent of a metal-binding domain. We examined the metal-binding properties of CRP isolated from chicken smooth muscle (cCRP) and from a bacterial expression system and observed that cCRP is a specific Zn-binding metalloprotein. Four Zn(II) ions are maximally bound to cCRP, consistent with the idea that each LIM domain coordinates two metal ions. From spectroscopic studies of Co(II)- and 113Cd(II)-substituted cCRP, we determined that each metal ion is tetrahedrally coordinated with cysteinyl sulfurs dominating the ligand types. One metal site within each LIM motif has tetrathiolate (S4) coordination, the second site may either be S4 or S3N1. The LIM motif represents another example of a specific Zn-binding protein sequence.

MeSH Terms
Amino Acid Sequence Animals Apoproteins/chemistry Avian Proteins Chickens Cloning, Molecular Cobalt/chemistry Consensus Sequence Magnetic Resonance Spectroscopy Metalloproteins/chemistry Molecular Sequence Data Muscle Proteins Proto-Oncogene Proteins c-myc/chemistry Recombinant Proteins/chemistry Sequence Alignment Zinc/chemistry
Chemicals
Apoproteins Avian Proteins Metalloproteins Muscle Proteins Proto-Oncogene Proteins c-myc Recombinant Proteins cysteine-rich protein, avian Cobalt Zinc
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Michelsen J W
Department of Biology, University of Utah, Salt Lake City 84132.
Schmeichel K L
Beckerle M C
Winge D R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.10.4404
Published
1993-05-15
Pages
4404-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46519
Subset
IM
Grants
NCI NIH HHS · CA42014 · United States
NIEHS NIH HHS · ES03817 · United States
NHLBI NIH HHS · HL41553 · United States
External Links
PubMed source DOI: 10.1073/pnas.90.10.4404 PMC Full Text View journal details
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