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PMID: 8501206 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation of specific DNA fragments of Mycobacterium avium and their possible use in diagnosis.

Journal of clinical microbiology ·Vol. 31 ·No. 5 ·1993-05-00 ·Pages 1048-54

Thierry D, Vincent V, Clément F, Guesdon JL

Abstract

We cloned and sequenced two DNA fragments (DT1 and DT6) from Mycobacterium avium serotype 2 for use in the identification of members of the M. avium-M. intracellulare complex (MAC). Reference strains of MAC belonging to serovars 1 to 28 were examined by using these DNA fragments as probes. The study revealed that the DT6 probe hybridized with DNAs from M. avium strains (serovars 1 to 6, 8 to 11, and 21), while the DT1 probe hybridized with DNAs from serovars 2, 3, 7, 12 to 20, and 23 to 25. DT1- and DT6-derived oligonucleotides were selected for use as primers in a polymerase chain reaction test. Amplification of the DT1 and DT6 sequences may provide the basis for a rapid and reliable assay for the detection of mycobacteria belonging to MAC.

MeSH Terms
Animals Base Sequence DNA Probes DNA, Bacterial/genetics,isolation & purification Humans Molecular Sequence Data Mycobacterium avium/classification,genetics,isolation & purification Mycobacterium avium Complex/genetics,isolation & purification Mycobacterium avium-intracellulare Infection/diagnosis Polymerase Chain Reaction Species Specificity
Chemicals
DNA Probes DNA, Bacterial
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Thierry D
Laboratoire de Prédéveloppement des Sondes, Institut Pasteur, Paris, France.
Vincent V
Clément F
Guesdon J L
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.31.5.1048-1054.1993
Published
1993-05-00
Pages
1048-54
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC262878
Subset
IM
Databases
GENBANK
L04542, L04543
External Links
PubMed source DOI: 10.1128/jcm.31.5.1048-1054.1993 PMC Full Text View journal details
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