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PMID: 8501034 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Characterization of the cobalamin (vitamin B12) biosynthetic genes of Salmonella typhimurium.

Journal of bacteriology ·Vol. 175 ·No. 11 ·1993-06-00 ·Pages 3303-16

Roth JR, Lawrence JG, Rubenfield M, Kieffer-Higgins S, Church GM

Abstract

Salmonella typhimurium synthesizes cobalamin (vitamin B12) de novo under anaerobic conditions. Of the 30 cobalamin synthetic genes, 25 are clustered in one operon, cob, and are arranged in three groups, each group encoding enzymes for a biochemically distinct portion of the biosynthetic pathway. We have determined the DNA sequence for the promoter region and the proximal 17.1 kb of the cob operon. This sequence includes 20 translationally coupled genes that encode the enzymes involved in parts I and III of the cobalamin biosynthetic pathway. A comparison of these genes with the cobalamin synthetic genes from Pseudomonas denitrificans allows assignment of likely functions to 12 of the 20 sequenced Salmonella genes. Three additional Salmonella genes encode proteins likely to be involved in the transport of cobalt, a component of vitamin B12. However, not all Salmonella and Pseudomonas cobalamin synthetic genes have apparent homologs in the other species. These differences suggest that the cobalamin biosynthetic pathways differ between the two organisms. The evolution of these genes and their chromosomal positions is discussed.

Related Genes
cbiA cbiB cbiC cbiD cbiE cbiF cbiG cbiH cbiK cbiL cbiM cbiN cbiO cbiP cbiQ cobS cobU
MeSH Terms
Amino Acid Sequence Base Sequence Biological Evolution Chromosome Mapping Cobamides/metabolism Conserved Sequence Gene Expression Regulation, Bacterial Genes, Bacterial/genetics Methylation Methyltransferases/metabolism Molecular Sequence Data Operon/genetics Promoter Regions, Genetic/genetics Protein Biosynthesis Salmonella typhimurium/genetics Sequence Homology, Amino Acid Vitamin B 12/biosynthesis
Chemicals
Cobamides cobinamide Methyltransferases Vitamin B 12
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Roth J R
Department of Biology, University of Utah, Salt Lake City 84112.
Lawrence J G
Rubenfield M
Kieffer-Higgins S
Church G M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.11.3303-3316.1993
Published
1993-06-00
Pages
3303-16
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC204727
Subset
IM
Grants
NIGMS NIH HHS · GM 34804 · United States
Databases
GENBANK
L12006
External Links
PubMed source DOI: 10.1128/jb.175.11.3303-3316.1993 PMC Full Text View journal details
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